出生前后慢性铝暴露对大鼠海马长时程增强及细胞内Ca^(2+)浓度的影响  

Effects of Chronic Aluminum Exposure from Prenatal to Postnatal on LTP and Ca^(2+) Concentration in Hippocampus of Young Rats

作　　者：刘鹏[1,2] 伟忠民[2] 南娟[1,3] 张占雀 时利德[1] 

机构地区：[1]中国医科大学基础医学院生理学教研室,沈阳110001 [2]辽宁医学院附属第一医院药品管理科,辽宁锦州121001 [3]天津医科大学总医院肺癌研究所,天津300052 [4]山西省芮城县人民医院内科,山西芮城044600

出　　处：《中国医科大学学报》2009年第7期484-486,507,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30371229)

摘　　要：目的研究出生前后慢性铝暴露对年轻大鼠海马长时程增强(LTP)及细胞Ca2+浓度的影响,进一步探讨铝损害学习与记忆的突触机制。方法对照组、0.2%Al组和0.4%Al组大鼠从孕期始分别自由饮用蒸馏水、Al3+浓度为15 mmol/L和30 mmol/L的A1C13水溶液。采用细胞外微电极记录法测定海马LTP;以Fura-2/AM为荧光指示剂,检测实施LTP诱导后海马神经元内Ca2+浓度的变化。结果与对照组相比,铝暴露组群体锋电位平均相对幅值增强率均明显下降(P<0.01),海马细胞内Ca2+浓度降低(P<0.05)。2个铝暴露组间Ca2+浓度的差异也有统计学意义(P<0.05)。结论出生前后慢性铝暴露损害了海马LTP的诱导与维持的机制之一可能是Ca2+浓度的降低。Objective To study the changes of LTP and Ca^2＋ concentration in hippocampus of young rats after chronic aluminum （Al） exposure of different concentration from prenatal to postnatal, to go deep into the synaptic mechanism of that aluminum impairing learning arid memory capacity. Methods The extracelluar microelectrode technique was used to monitor the LTP. Ca^2＋ concentration in hippocampal was measured by the technique of Fttra-2/AM calcium ions fluorescence indicator. Results The PS mean amplitudes relative to the baseline values after HFS in control group were higher than that in Al^3＋ exposed groups. Compared with the control group, the Ca^2＋ concentration was significantly decreased in the Al^3＋ exposed groups （P〈 0.05）. Conclusion Aluminum exposure from prenatal to postnatal can damage the Ca^2＋ concentration in hippocampal, consquentely impair the induction and maintenaee of LTP in hippocamous of young rats.

关 键 词：铝暴露 长时程增强 钙离子 

分 类 号：R332.81[医药卫生—人体生理学]