大鼠急性脊髓损伤后神经细胞凋亡及caspase-3、AIF的表达  

Neural Cell Apoptosis and the Expression of Caspase-3,Apoptosis-inducing Factor after Acute Spinal Cord Injury

作　　者：崔开[1] 韩亚新[1] 董君博[1] 孔冉冉[1] 丛琳[1] 吕荼[1] 屠冠军[1] 

机构地区：[1]中国医科大学附属第一医院骨科,沈阳110001

出　　处：《中国医科大学学报》2009年第7期508-510,532,共4页Journal of China Medical University

基　　金：辽宁省教育厅高校科研基金资助项目(2004C044)

摘　　要：目的研究大鼠急性脊髓损伤后神经细胞凋亡及caspase-3、凋亡诱导因子(AIF)的表达。方法将78只成年健康Sprague-Dawley(SD)大鼠按Nystrom法建立大鼠脊髓(T8、T9)急性压迫损伤模型,HE染色观察脊髓组织病理学变化,免疫组化测定各时间点AIF和caspase-3的表达变化,原位末端脱氧核糖核酸转移酶介导的脱氧尿苷三磷酸(dUTP)标记法(TUNEL法)检测神经细胞的凋亡水平。结果免疫组化结果显示正常及假手术组大鼠脊髓神经细胞caspase-3,AIF,TUNEL阳性细胞较少,脊髓损伤后1 d,AIF阳性细胞数明显增多(P<0.05),5 d表达减弱。caspase-3阳性细胞数在脊髓损伤后8 h明显增多,3 d达高峰(P<0.01),7 d表达减弱。TUNEL阳性细胞数也在8 h明显增多,3 d达高峰(P<0.01),7 d表达减弱。结论脊髓损伤后存在广泛的细胞凋亡现象,caspase-3、AIF均参与了细胞凋亡的调节。Objective To study the apoptosis of the neural cells and the changes of caspase-3,AIF＇s expression after acute spinal cord injury in rats. Methods The spinal cord injury of the healthy adult SD rats （78） were induced with Nystmm＇ s way by the mederate compression at the level of T8 and T9 spinal cord.HE method was used to detect the pathologic change of spinal cord. The expression of caspase-3 and AIF was detected by immunohistochemical study. Besides, by using the DUTP nick and labeling （TUNEL） methods which was mediated by terminal deoxynucleotidyl transferase to detect the level of the apoptosis of neural cells. Results There was less expression of AIF,caspase-3 and TUNEL-positive cells in normal and sham operated spinal cord as detected by immunohistochemical study. The number of positive cells of AIF increased clearly 1 day and then reached a peak, but it was rarely seen 5 days after spinal injury. The expression of caspase-3 increased obviously 8 hours after spinal injury, then reached a peak 3 days after spinal injury, but it decreased 7 days after spinal injury. The number of positive cells of TUNEL also increased obviously 8 hours after spinal injury, and then reached a peak on 3 day, and also decreased 7 days after spinal injury. Conclusion There is apoptosis of the neural cells after acute spinal cord injury, and AIF, caspase-3 may be involved in the regulation of the apoptosis of neural cells after acute spinal cord injury.

关 键 词：脊髓损伤 细胞凋亡 天冬氨酸特异性半胱氨酸蛋白酶3 凋亡诱导因子 

分 类 号：R681.5[医药卫生—骨科学]