大豆异黄酮对去睾丸大鼠血糖及血脂的调节作用研究  

Effects of Soy Isoflavone on Serum Glucose and Lipid in Orchiectomized Rats

作　　者：李丽娜[1] 张云波[1] 赵新宇[1] 刘敏[1] 赵倩[1] 那晓琳[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生学教研室,黑龙江哈尔滨150081

出　　处：《环境与健康杂志》2009年第10期850-852,共3页Journal of Environment and Health

基　　金：国家自然科学基金资助项目(30671755);黑龙江省卫生厅资助项目(2006-300)

摘　　要：目的研究环境内分泌干扰物——植物雌激素大豆异黄酮(soy isoflavones,SIF)对去睾丸雄性大鼠血糖及血脂的调节作用。方法将54只健康7周龄清洁级雄性Wistar大鼠按体重随机分为6组,分别为假手术组(SHAM)、去睾丸对照组(ORCH)、低剂量大豆异黄酮组(L-SI)、中剂量大豆异黄酮组(M-SI)、高剂量大豆异黄酮组(H-SI)和雌激素对照组(EC),每组9只。用1.5%戊巴比妥钠按40mg/kg腹腔注射麻醉,除假手术组进行单纯开腹手术外,其余5组均摘除睾丸。高脂饲料喂养4周,其中,低、中、高剂量大豆异黄酮组每kg高脂饲料分别加入大豆异黄酮1、3、9g,雌激素对照组每kg高脂饲料中加入己烯雌酚0.45mg。在第2、4周分别检测大鼠胰岛素、空腹血糖(FBG)及血脂[甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)]水平。结果去除睾丸后大鼠的体重显著下降,差异均有统计学意义(P<0.05)。与雌激素对照组比较,假手术组、去睾丸对照组和高、中、低剂量大豆异黄酮组大鼠体重均较高,差异有统计学意义(P<0.05)。大鼠体重多随着大豆异黄酮染毒剂量的升高而降低。与去睾丸对照组相比,第4周高、中、低剂量大豆异黄酮组大鼠血糖均显著下降,中、高剂量大豆异黄酮组大鼠胰岛素水平显著升高,差异均有统计学意义(P<0.05)。大豆异黄酮和雌激素可显著降低大鼠TC水平(P<0.05),而对LDL无明显影响(P<0.05),不能改善大鼠HDL水平(P<0.05),对TG有一定的降低作用,但差异无统计学意义(P>0.05)。结论大豆异黄酮可通过提高去睾丸大鼠体内胰岛素水平而发挥降血糖作用,可显著降低TC水平,对TG具有一定的调节作用,但对HDL和LDL无明显影响。Objective To investigate the effects of environmental endocrine disruptors-phytoestrogen soybean isoflavone on serum glucose and lipid in rats with orchiectomy. Methods Fifty-four healthy clean male Wistar rats aged 7 weeks were randomly divided into 6 groups, 9 in each group, according to their weight and named as the groups of SHAM, ORCH, L-SI, M-SI, H-SI and EC. The rats were injected intraperitoneally with 1.5% sodium pentobarbital according to 40 mg/kg,and they were either shamoperated or orchiectomized. The rats were fed on high fat diet for 4 weeks. There was 1,3,9 g soybean isoflavone and 0.45 mg estrobene per kilogram high fat diet in L-SI, M-SI, H-SI and EC respectively. At the 2nd and 4th week, serum insulin, fasting blood glucose and lipid （TG,TC,HDL and LDL） were detected. Results The weights of rats with orchiectomy was significantly decreased compared with sham-operated rats, and compared with the EC group, the weights of other groups＇ rats were significantly higher. The weight was decreased along with the increase of the dose of soy isoflavone. At the end of 4 weeks, SIF significantly decreased serum glucose compared with ORCH, and the insulin level was significantly increased in M-SI and H-SI group. SIF and estrogen could significantly decrease TC, but they did not have obvious effect on LDL. SIF could not improve HDL level in rats with orchiectomy. Even though there was no statistic difference,the TG levels of SIF groups were deceased. Conclusion SIF can decrease serum glucose level via raising insulin level in rats with orchiectomy. SIF can decrease serum TC level significantly and regulate TG level while there is no influence on HDL and LDL.

关 键 词：大豆异黄酮 去睾丸大鼠 血糖 血脂 

分 类 号：R994.6[医药卫生—毒理学]