甲醛致A549细胞DNA-蛋白质交联及其修复效应  

Effects on DNA-Protein Crosslinks Induced by Formaldehyde in A549 Cell Lines

作　　者：江中发[1,2] 胡建国 张玲[1] 李宁[1] 袁春[1] 张本延[1] 

机构地区：[1]武汉科技大学医学院预防医学系,湖北武汉430065 [2]湖北省疾病预防控制中心,湖北武汉430079 [3]杭州经济开发区白杨街道社区卫生服务中心,浙江杭州310018

出　　处：《环境与健康杂志》2009年第10期872-874,共3页Journal of Environment and Health

摘　　要：目的探讨甲醛诱导DNA-蛋白质交联作用及其修复效应。方法于37℃条件下,以不同浓度的液态甲醛(0、50、100、200、400、800、1600μmol/L)对培养人类肺肿瘤(A549)细胞(对数生长期)染毒1h后,检测A549细胞的DPC系数。于37℃下对100μmol/L液态甲醛染毒1h的A549细胞的DPC修复0、6、12、18、24h,设1个空白对照组,并检测A549细胞的DPC系数。采用KCl-SDS沉淀法检测DPC系数。结果与溶剂对照组(0μmol/L)比较,100、200、400、800、1600μmol/L甲醛染毒组A549细胞的DPC系数均较高,差异有统计学意义(P<0.05)。50μmol/L甲醛染毒组A549细胞的DPC系数与溶剂对照组相比虽有升高趋势,但差异无统计学意义(P>0.05)。经过100μmol/L的液态甲醛给A549细胞染毒后,0h修复组DPC系数高于空白对照组,差异有统计学意义(P<0.05)。液态甲醛诱导A549细胞产生的DPC经过6、12和18h修复后,DPC系数与0h修复组相比虽有下降趋势,但差异均无统计学意义(P>0.05);经过24h修复后,DPC系数低于0h修复组,差异均有统计学意义(P<0.05),但与空白对照组相比,差异无统计学意义(P>0.05)。结论较高浓度的甲醛可以明显诱导DPC形成,甲醛所致的DPC可以得到修复。Objective To explore the DNA-protein crosslinks （DPC） and its repair induced by formaldehyde （FA）. Methods A549 cells were used as the experimental materials exposed to FA at different concentrations of liquid FA （0,50,100,200,400,800, 1 600μmol/L） in 37℃ for 1 h. The effect of its repair at 0,6, 12, 18,24 h after exposed to FA of 100μmol/L for 1 h was detected. KCl-SDS assay was used to detect the coefficient of DPC. Results Higher concentration of liquid FA （100μmol/L or above） could increase the coefficient of DPC （P〈0.05）.Coefficient of DPC which were exposed to lower concentration of liquid FA （50 μmol/L） was a little higher compared with the control group （P〉0.05）. DPC induced by liquid FA of 100 μmol/L could be repaired in and be restored to the level of the control group around 24 h （P〉0.05）. Conclusion The study suggests that the coefficient of DNA-protein crosslinks can be increased significantly by higher concentrations of FA in A549 cell lines. DPC induced by FA could be repaired.

关 键 词：甲醛 细胞培养 DNA-蛋白质交联 修复 

分 类 号：R994.6[医药卫生—毒理学]