头风愈滴丸HPLC指纹图谱的研究  

HPLC fingerprint chromatography of Toufengyu Dropping Pills

作　　者：耿志鹏[1] 冯瑜娟[1] 王毓杰[1] 张艺[1] 马逾英[2] 孟宪丽[2] 

机构地区：[1]成都中医药大学民族医药学院,四川成都611137 [2]成都中医药大学药学院,四川成都611137

出　　处：《中成药》2009年第9期1316-1319,共4页Chinese Traditional Patent Medicine

基　　金：十一五国家科技支撑计划(2007BAI40B02);十一五国家科技支撑计划(2006BAI06A18-15)

摘　　要：目的:建立复方头风愈滴丸(白芷,川芎,绿茶)的HPLC指纹图谱分析方法。方法:色谱柱:Hypersil ODS2 C18(4.6mm×250 mm,5μm);流动相:乙腈-0.1%三氟乙酸水溶液(梯度洗脱);流速:1.0 mL/min;柱温:30℃;检测波长:254nm。结果:通过对10批自制制剂的测定,该方法具有很好的重复性,制剂中共有峰分离良好,标定了22个共有峰,并通过对照品比对鉴定出其中的9个成分。即咖啡因,EGCG,阿魏酸,氧化前胡素,欧前胡素,珊瑚菜素,川芎内酯,异欧前胡素,水合氧化前胡素。结论:该方法准确、可靠,可以为头风愈滴丸的质量控制提供较全面的信息。AIM： To establish a method of fingerprint analysis of Toufengyu Dropping Pill（Radix angelicae dahuricae,Rhizoma chuanxiong and green tea） by HPLC. METHODS： Hypersil ODS2 C18（4.6 mm×250 mm,5 μm） column was used,with mixtures of 0.1%TFA solution and acetonitrile as mobile phase in a gradient mode.The flow rate was 1.0 mL/min,the column temperature was at 30 ℃,detection wavelength was at 254 nm. RESULTS： HPLC fingerprint determination with 22 common peaks gained from 10 batches of self-made Tonfengyu Dropping Pill, there were 9 components identified, including caffeine, EGCG, femlic acid, oxypeucedanin, im- peratorin, phellopterin, cnidilin, isoimperatorin and oxypeucedanin hydrate. CONCLUSION： This method is accurate, reliable and can provide more information for the quality control of Tonfengyu Dropping Pills.

关 键 词：头风愈滴丸 HPLC 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]