海南砂仁挥发油对实验性溃疡性结肠炎小鼠抗氧化和抗NO自由基作用(英文)  

Antioxidative and antinitrosative effects of volatile oil from A.longiligulare T.L.Wu on ulcerative colitis mice

作　　者：赵锦[1] 朱毅[1] 董志[2] 陈国彪[1] 刘春[1] 

机构地区：[1]海南省药品检验所,海南海口570216 [2]重庆医科大学药学院药理教研室,重庆市生物化学及分子药理学重点实验室,重庆630000

出　　处：《中成药》2009年第9期1334-1338,共5页Chinese Traditional Patent Medicine

基　　金：Supported by 2007 National Key Technology R&D program(2007BAI27B06);2006 Key Science and Technology Projects of Hainan Province(06207)

摘　　要：目的:考察海南砂仁挥发油的抗氧化及抗NO作用,对海南砂仁挥发油抗实验性溃疡性结肠炎(UC)作用机制进行初步探讨。方法:自由饮用4%右旋葡聚糖硫酸钠(DSS)溶液建立UC模型。生化法测定正常对照组、模型对照组、海南砂仁挥发油高、中、低剂量组及SASP阳性药对照组小鼠肠组织中SOD、MDA以及NO水平;免疫组化考察各组的iNOS表达。结果:海南砂仁挥发油中高剂量给药可以显著降低模型组肠组织中MDA水平,显著升高SOD水平,并且可显著降低模型小鼠NO及iNOS水平。结论:海南砂仁挥发油具有抗氧化及抗NO作用,其可通过抑制iNOS表达,减少NO过量生成,是海南砂仁挥发油发挥抗UC的作用机制之一。AIM ： To investigate antioxidative and antinitrosative effects of volatile oil from A. longiligulare T. L. Wu on dextran sulfate sodium（DSS）-induced ulcerative colitis mice. METHODS： Balb/c mice were fed with 4% DSS solution for 7 d to induce ulcerative colitis. Using biochemical method, the activity of antioxidative enzyme SOD, MDA and NO were determined in normal, model, SASP and three mouse＇ s groups with low, moderate and high volatile oil from A. longiligulare T. L. Wu respectively. At the same time, the activity of iNOS was also measured by immunohistopathology. RESULTS： The concentration of MDA and NO were reduced and SOD increased significantly in high-and moderate-volatile oil groups compared with those in the model group. The activity of iNOS was reduced significantly in high-and moderate-volatile oil groups compared with those in the model group. The results demonstrated that the expression of iNOS was significantly inhibited in DSS-induced ulcerative colitis mice after being treated with high or moderate-dosage volatile oil. CONCLUSION： Volatile oil from A. longiligulare T. L. Wu has antioxidative and antinitrosative effects which may be one of the mechanism for treating UC.

关 键 词：海南砂仁 挥发油 溃疡性结肠炎 抗氧化 NO INOS DSS 小鼠 

分 类 号：R285.6[医药卫生—中药学]