氯化两面针碱对肝、肾的毒性及酸性成纤维细胞生长因子对其的保护作用研究  

Protection in vitro of aFGF on human liver cell L-02 and human kidney cell 293 caused by nitidine chloride

作　　者：韦敏[1] 刘丽敏[2] 李丹妮[2] 刘华钢[2] 

机构地区：[1]广西医科大学附属第五医院,广西柳州545005 [2]广西医科大学,广西南宁530020

出　　处：《中成药》2009年第9期1338-1341,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(30850010);广西科学研究与技术开发计划项目(桂科改0630002-E)

摘　　要：目的:观察氯化两面针碱在体外对人胚肝细胞L-02,人胚肾细胞293的毒性作用,及酸性成纤维细胞生长因子(aF-GF)对氯化两面针碱引起的肝、肾细胞损伤的保护作用。方法:采用MTT法检测不同浓度氯化两面针碱对人肝、肾细胞的毒性,及加入aFGF后对肝、肾细胞的IC50值的影响。采用紫外分光光度法测定培养上清液中的SOD、MDA、LDH值。结果:aFGF可明显提高人胚肝细胞L-02、人胚肾细胞293的IC50值。aFGF保护组与氯化两面针碱损伤组的SOD、MDA和LDH测定结果有显著性差异(P<0.05)。结论:氯化两面针碱对人胚肝细胞L-02和人胚肾细胞293有一定毒性,加入aFGF后,可明显减轻氯化两面针碱对人胚肝细胞L-02和人胚肾细胞293的毒性作用。AIM： To detect the inhibition effect of nitidine chloride on proliferation of human liver cell L-02 and human kidney cell 293 and the protective effect of acidic fibroblast growth factor（aFGF） on human liver cell L- 02 and human kidney cell 293 damaged by nitidine chloride in vitro. METHODS： The MTr assay was used to assess the proliferation of human liver cell L-02 and human kidney cell 293 treated with nitidine chloride. The contents of SOD and MDA and LDH in cultural supemate were measured by ultraviolet spectrophotometry. RESULTS ： Nitidine chloride inhibited the proliferation of human liver cell L-02 and human kidney cell 293 in a dose-dependent manner. CONCLUSION： Nitidine chloride has certain toxicity on human liver cell L-02 and human kidney cell 293. aFGF could protect human liver cell L-02 and human kidney cell 293 damaged by nitidine chloride.

关 键 词：氯化两面针碱 人胚肝细胞L-02 人胚肾细胞293 毒性 酸性成纤维细胞生长因子 

分 类 号：R285.6[医药卫生—中药学]