蝉花菌丝体粉胶囊的质量标准研究  

Quality standard of Chanhua Junsitifen Capsule

作　　者：周靖[1] 张乐乐[1] 邵燕[1] 孔德云[1] 

机构地区：[1]上海医药工业研究院中药研究室,上海200040

出　　处：《中成药》2009年第9期1379-1382,共4页Chinese Traditional Patent Medicine

摘　　要：目的:制定蝉花菌丝体粉胶囊的质量标准。方法:采用TLC法对蝉花菌丝体粉胶囊中的氨基酸、腺嘌呤、腺苷和尿苷进行定性鉴别;采用HPLC法对腺苷进行了含量测定,色谱条件:采用Inertsil C18(4.6 mm×250 mm,5μm)色谱柱,流动相为甲醇-0.05 mol/L磷酸二氢钾水溶液(15∶85),流速:1.0 mL/min,于260 nm处用外标法检测。结果:腺苷在0.007 475～2.99μg范围内线性关系良好,r=0.999 9,平均回收率为99.60%,RSD为0.62%。结论:建立的方法简便,准确,重复性好,能有效控制蝉花菌丝体粉胶囊的质量。AIM： To establish the quality standard for Chanhua Junsitifen Capsule（Paecilomyces cicadae（Miquel） samson）. METHODS： Amino acids,adenine,adenosine,uridnine in Chanhua Junsitifen Capsule were identified by TLC and adenosine was determined by HPLC.Chromatographic conditions were adopted as follows：co-lumn： Inertsil C_18（4.6 mm×250 mm,5 μm）,mobile phase： MeOH-0.05 mol/L KH_2PO_4 solution（15∶85）,wavelength： 260 nm,flow rate： 1.0 mL/min. RESULTS： The average recovery was 99.60 % and RSD was 0.62 % and the linear range of adenosine was in the range 0.007 475 - 2.99 μg. CONCLUSION ： This method is simple, rapid with a good reproducibility. This method can be used for the quality control of Chanhua Junsitifen Capsule.

关 键 词：蝉花菌丝体粉胶囊 薄层鉴别 腺苷 含量测定 

分 类 号：R927.2[医药卫生—药学]