寻常型天疱疮患者一级亲属中Dsg3诱导T淋巴细胞的增殖  

Proliferative Response of T lymphocyte to Dsg3 in First-degree Relatives of the Patients with Pemphigus Vulgaris

作　　者：魏仲香[1] 贾秀坤[2] 胡婕[1] 周鸿波[1] 季永智[1] 梁再赋[2] 耿龙[1] 

机构地区：[1]中国医科大学附属第一医院皮肤性病科,沈阳110001 [2]中国医科大学实验技术中心一部,沈阳110001

出　　处：《中国医科大学学报》2009年第8期601-603,共3页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30471567);长江学者和创新团队发展项目(IRT0760)

摘　　要：目的研究寻常型天疱疮(PV)特异性抗原桥粒芯糖蛋白3(Dsg3)刺激下PV患者一级亲属T淋巴细胞的增殖情况,探讨PV的发生机制。方法Dsg3刺激PV患者一级亲属外周血单个核细胞(PBMC)体外培养3 d,BrdU掺入流式细胞术胞内染色,检测Dsg3特异性CD3+、CD3+CD4+、CD3+CD8+T细胞的增殖情况。结果PV抗体阳性亲属中PBMC经Dsg3刺激培养者与未经Dsg3刺激培养者比较,CD3+CD4+T细胞增殖率显著升高[(2.79±1.05)%vs(1.85±0.44)%,P<0.05],PV抗体阳性亲属CD3+CD4+T细胞增殖率明显高于正常对照者[(2.79±1.05)%vs(1.25±0.71)%,P<0.05]。结论PV患者一级亲属中PV抗体携带者体内存在Dsg3自身反应性CD3+CD4+T淋巴细胞,Dsg3释放可能在PV启动中起重要作用。Objective To investigate the characteristics of T lymphocyte proliferation in first-degree relatives of the pemphigus vulgaris（PV）patients under the stimulation of Dsg3 and clarify the mechanism of the Dsg3-specific autoimmunity of PV.Methods We examined Dsg3 reactive peripheral blood T-cell subsets CD3＋,CD3＋CD4＋,CD3＋CD8＋ T cells by BrdU incorporation assay and flow cytometry.Results After stimulated by Dsg3 antibody,the proliferative ratio of CD3＋CD4＋T lymphocytes in first-degree relatives with PV antibody positive was significantly increased [ （2.79±1.05）% vs （1.85±0.44）%, P 〈 0.05 ]. Dsg3-speciilc CD3＋CD4＋ T lymphocyte proliferation on PV-antibody- positive first degree relatives was significantly higher than that of healthy controls [ （2.79±1.05）% vs （ 1.25±0.71 ）%, P 〈 0.05 ]. Conclusion The first-degree relatives of PV patients who carry the PV antibodies have Dsg3 autoreative T lymphocytes. The release of Dsg3 may play important roles in the initiation of PV.

关 键 词：寻常型天疱疮 一级亲属 T淋巴细胞 增殖 流式细胞术 

分 类 号：R758.66[医药卫生—皮肤病学与性病学]