安徽省2007～2008年志贺氏菌流行株菌型分布及脉冲场分子分型  

Bacteria-type Distribution and Molecular Typing of Bacillary Dysentery Epidemic Strains by Pulsed-field in Anhui Province in 2007～2008

作　　者：胡守奎[1] 胡万富[1] 陆美娟[1] 撒楠[1] 袁媛[1] 张钧[1] 

机构地区：[1]安徽省疾病预防控制中心,安徽合肥230061

出　　处：《安徽预防医学杂志》2009年第5期342-344,共3页Anhui Journal of Preventive Medicine

摘　　要：目的了解安徽省志贺氏菌的流行菌型,并建立脉冲场凝胶电泳(PFGE)分子分型方法,为菌痢防控提供理论基础。方法对22株志贺氏菌进行血清分型、药物敏感试验和PFGE试验。结果22株志贺氏菌血清分型:B群19株,占总数86.5%;C群1株,占总数的4.5%;D群2株,占总数的9.0%。成功建立了菌痢的PFGE分子分型方法,并将我省22株流行株分为若干带型。试验结果进一步作聚类分析。结论安徽省菌痢流行株以福氏为主;安徽省PFGE分子分型方法和初步带型数据库对提高细菌检测水平有一定意义。Objective To understand the prevalence of Shigella bacteria type in Anhui province and establish pulsed - field gel electrophoresis （ PFGE ） molecular typing method so as to provide a theoretical basis for the prevention and control of bacillary dysentery. Methods Carry out serotyping test, PFGE test and drug sensitivity test of 22 strains Shigella. Results Of 22 strains Shigella, B group 19, accounting for 86.5% ; C group 1, accounting for 4.5 % ; D group 2, accounting for 9.0% of the total number. Successfully established the PFGE molecular typing methods ; and 22 epidemic strains in our province is divided into a number of bands. The results would further cluster analysis. Conclusions The prevalence type of bacillary dysentery in our province was B group; PFGE molecular typing methods and a preliminary zone database were established in Anhui CDC which would help to detect and identify bacillary dysentery epidemic strains.

关 键 词：志贺氏菌 流行株 脉冲场 分子分型 

分 类 号：R516.4[医药卫生—内科学]