西藏八角莲种胚愈伤诱导的初探  

Primary Study on the Callus Inducement from Embryo of Dysosma tsayuensis Ying

作　　者：杨爽[1] 王勇 陈芝兰[1] 何建清[1] 田发益[1] 

机构地区：[1]西藏大学农牧学院/生物技术中心,西藏林芝860000 [2]西藏林芝地区农牧局,西藏林芝860000

出　　处：《北京农学院学报》2009年第3期63-65,共3页Journal of Beijing University of Agriculture

基　　金：西藏自治区科技厅资助项目(200422)

摘　　要：以西藏八角莲成熟种子为材料,研究了外植体的不同处理方法、激素浓度及光照条件对愈伤诱导的影响。结果表明,在暗培养条件下,以带种胚的种子段为外植体,在MS+6-BA 2.0 mg/L+NAA 0.2 mg/L培养基上可以获得诱导率较高的愈伤组织,诱导率为66.7%。Using the mature seeds of Dysosma tsayuensis Ying as material,the effects of different methods on dealing with the explants,hormone concentration and illumination conditions on the callus inducement of Dysosma tsayuensis Ying were studied.The results showed that in the dark environment,when the part of seed with the embryo was chosen as the explants callus,the inducement rate was higher in the medium of MS ＋6-BA 2.0 mg/L ＋ NAA 0.2 mg/L than in other media,and the callus inducement rate was 66.7%.

关 键 词：西藏八角莲 激素 愈伤组织 诱导 

分 类 号：S567.239.01[农业科学—中草药栽培]