一种基于液相色谱-质谱技术进行血清代谢组学研究的方法:从代谢指纹到潜在标志物  

A metabonomics approach based on liquid chromatography-mass spectrometry:from metabolic profiling to potential biomarker

作　　者：陈静[1] 单圆鸿[1] 严沁[2] 赵素敏[1] 尹沛源[1] 赵欣捷[1] 路鑫[1] 席晓薇[2] 万小平[2] 许国旺[1] 

机构地区：[1]中国科学院大连化学物理研究所,中国科学院分离分析重点实验室,大连116023 [2]上海交通大学附属第一人民医院妇产科,上海200080

出　　处：《中国科学（B辑）》2009年第10期1268-1276,共9页Science in China(Series B)

基　　金：国家高技术研究发展计划(编号:2006AA02Z342);蛋白质科学重大研究计划(编号:2007CB914701)项目资助

摘　　要：本文描述了一种基于液相色谱-质谱技术(LC-MS)的代谢组学发现疾病潜在标志物的方法.该方法利用LC-MS获得代谢指纹图谱,并通过多种统计分析方法对产生的海量数据进行分析,最终筛选出潜在标志物.数据分析过程包括:通过归一化、修正80%规则、数据集分割和数据缩放等方法对数据集进行预处理;通过正交校正的偏最小二乘(OPLS)模式识别方法对样品进行分型;根据模型的变量重要性因子(VIP值)、非参数检验结果和z值筛选潜在标志物.以宫颈癌血清样本为例,应用上述方法,15个变量被确认为潜在标志物,操作者接受曲线(ROC)下的面积为0.667～0.956.经过相关性分析和结构鉴定,发现这15个变量来自9个化合物.其中7个化合物被鉴定为色氨酸、硬脂酸、花生四烯酸、溶血磷脂酰胆碱(0:0/16:0,16:0/0:0,18:1/0:0和18:0/0:0),说明在宫颈癌中花生四烯酸和溶血磷脂酰胆碱的代谢发生异常.A LC-MS based metabonomics approach for potential biomarkers screening is described. Metabolic profiles were acquired by LC-MS technology, and potential biomarkers were filtered by multiple statistical methods. Data pretreatment includes data normalization and scaling, corrected 80% rule, and dataset division. According to metabolic patterns, cervical cancer and health group were separated by OPLS. Potential biomarker screening was performed according to VIP value, significant test and z-score. As a result, fifteen variables were considered as potential biomarker, and their AUC were 0.667-0.956. The fifteen variables correspond to nine compounds, seven of which were identified as tryptophan, stearic acid, arachidonic acid, and lysoPC （0：0/16：0, 16：0/0：0, 18： 1/0：0, 8 ： 0/0 ： 0）. It can be concluded that abnormal metabolism of arachidonic acid and lysoPC happened in cervical cancer.

关 键 词：代谢组学 液质联用 宫颈癌 肿瘤标志物 

分 类 号：R341[医药卫生—基础医学]