清纤方对肝纤维化大鼠转化生长因子β_1(TGF-β_1)表达的影响  

Effects of Qingxian Recipe on Expression of Transforming Growth Factors β_1 in Rats with Liver Fibrosis

作　　者：张林军[1] 刘军[1] 马洪波[1] 白朝辉[1] 常庚[2] 杨芸芳[3] 张明辉[4] 刘静[4] 董尚普[5] 

机构地区：[1]河北体育学院医院,河北石家庄050061 [2]河北医科大学第二医院,河北石家庄050000 [3]乐仁堂医药集团股份有限公司,河北石家庄050011 [4]河北医科大学第一医院,河北石家庄050031 [5]河北医科大学中医学院,河北石家庄050091

出　　处：《中国中医基础医学杂志》2009年第9期663-664,667,共3页JOURNAL OF BASIC CHINESE MEDICINE

摘　　要：目的:探讨清纤方抗肝纤维化的治疗机制。方法:利用CCl4制备肝纤维化大鼠模型,分为正常组、模型组、清纤方组、秋水仙碱组。治疗8周后,用HE和Masson染色观察肝纤维化的形成,免疫组化和RT-PCR检测肝组织中TGF-1βmRNA和蛋白的表达。结果:TGF-β1蛋白和mRNA的表达在治疗组均明显减少。结论:清纤方能有效减轻肝纤维化大鼠的肝脏损伤及纤维化程度,其机制可能与抑制肝内TGF-1β的表达有关。Purpose： To explore the therapeutic mechanism of Qingxianfang for anti-hepatic fibrosis. Method： Prepare hepatic fibrosis models of bandicoots and divide them into groups marked ＂normal＂, ＂control＂, ＂Qingxianfang＂ and ＂colchicines＂ by making use of CCl4. After eight weeks of medical treatment, an observation of hepatic fibrosis is carried on by using HE and Masson pigmentation, immunohistochemistry and RT-PCR checking of TGF-β1, mRNA and protein expression in hepatic tissue. Result ： The expression of TGF-β1 protein and mRNA reduced clearly in treatment group. Conclusion ： Qingxianfang is effective for easing the liver damage and hepatic fibrosis condition of bandicoots. The mechanism may be relative to the restraining of TGF-β1 expression inside the liver.

关 键 词：肝纤维化 复合物 清纤方 TGF-1β 

分 类 号：R285.5[医药卫生—中药学]