加味小柴胡汤对顺铂诱导的BRL细胞相关凋亡蛋白表达的影响  

Effects of Modified Xiao Chaihu Decoction on Proteins Expressions of Cisplatin-induced Apoptosis Related to BRL Cells

作　　者：刘应柯[1] 李合国[2] 宫璀璀[1] 

机构地区：[1]解放军第153中心医院,河南郑州450042 [2]河南中医学院第一附属医院,河北郑州450042

出　　处：《中国中医基础医学杂志》2009年第9期665-667,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：郑州市科技攻关计划(052SGYS33207);获2007年河南省科技进步二等奖

摘　　要：目的:探讨加味小柴胡汤对顺铂诱导的大鼠肝BRL细胞凋亡蛋白表达的调控作用。方法:将培养的BRL细胞分为空白组、顺铂组、实验1组(加味小柴胡汤大剂量)、实验2组(小剂量)4组。采用MTT、TUNEL、DNA琼脂糖凝胶电泳、免疫组化、完整细胞蛋白质斑点印迹等方法,检测各组细胞存活率、凋亡率及细胞相关凋亡蛋白的变化。结果:顺铂组细胞凋亡率比空白组明显升高,Rb、p21、Caspase-3、Bax、wp53蛋白表达上调;存活率显著降低,Bcl-2、mp53表达下调;实验1组、2组凋亡率明显较顺铂组降低,存活率升高,其他各指标皆与顺铂组有明显差异,1组作用更明显。结论:加味小柴胡汤可使顺铂诱导的BRL细胞存活率升高,凋亡率降低,对顺铂诱导BRL细胞凋亡蛋白的异常表达有调控作用。Objective： To approach regulating effect on Cisplatin induced rat liver BRL-cell＇ apoptosis proteinum express to added small Bupleuri Decoction. Method：We deployed method on MTT,TUNEL, DNA agarose gel electrophoresis, immunohistochemistry, complete cell protein spot blotting and so on to detect change for blank group, Platinol group, experiment 1 group, （added small Bupleuri Decoction large dose and 2 （added small Bupleuri Decoction small dose ） of cultivated BRL cell＇ cell survival rate, apoptosis rate and cell＇ apoptosis proteinum express. Results： The lower and higher of Cisplatin group cell surviva rate and apoptosis rate than blank group, experiment group land 2 all there are obviously difference; Cisplatin group cell Rb, p21, Caspase-3, Bax, wp53 proteinum obviously up-regulater and Bcl-2, mp53 obviously down regulater than blank group; experiment group 1 and 2 every index was near to blank group, the obviously differer than Cisplatin group. Conclusion： The added small Bupleuri Decoction can step up Cisplatin induced BRL cell survival rate and cut down apoptosis rate; can regulate on Cisplatin induced BRL-cell＇ apoptosis proteinum anormal express.

关 键 词：顺铂 加味小柴胡汤 BRL细胞 存活率 凋亡率 凋亡蛋白 

分 类 号：R285.5[医药卫生—中药学]