一株产碱性磷酸酶细菌的分离、鉴定及其产酶特性研究  

Isolation and Identification of an Alkaline Phosphatase-producing Bacterium and Its Enzymatic Properties

作　　者：庄百川[1] 陈海江[1] 徐晓红[1] 黄冰 贾小明[1] 

机构地区：[1]浙江大学生命科学学院,杭州310058

出　　处：《科技通报》2009年第5期593-598,共6页Bulletin of Science and Technology

基　　金：浙江省科技厅重点项目(2008C13014-3)

摘　　要：从土壤中分离、筛选到一株产碱性磷酸酶的菌株HB1,并且该菌株产生的碱性磷酸酶具有较好的热稳定性。根据形态学观察、生理生化特征,DNA中(G+C)mol%测定和16S rDNA序列同源性分析表明该菌为Bacillus licheniformi(地衣芽孢杆菌)。调节基础培养基起始pH为7.5,添加Mn2+100 mM/L,发酵24 h,可使HB1菌株细胞内外酶活分别达到5.3 U/mL和5.9 U/mL。A strain HB1 producing-alkaline phosphatase with good thermostability was screened from soil.Strain HB1 was identified as Bacillus licheniformi basis on morphological and physiological characteristics,（G＋C）mol% of DNA as well as sequence analysis of 16S rDNA of strain HB1.The alkaline phosphatase activity of strain HB1 was peaked 5.9 U / mL（intracellular）and 5.3 U/mL（extracellular）at pH 7.5 of medium with 100 mM / L MnSO4 after 24h of ferment.

关 键 词：碱性磷酸酶 芽孢杆菌 鉴定 

分 类 号：Q935[生物学—微生物学]