柴葛口服液(II)对大鼠肝纤维化作用的实验研究  

Experimentatal study on antihepatic fibrosis effect of Chaige oral liquid(II) in wistar mice

作　　者：艾继周[1] 王刚[2] 刘彬[2] 白华[3] 

机构地区：[1]重庆医药高等专科学校,重庆400030 [2]重庆医科大学附属儿童医院临床药学研究室,重庆400014 [3]宁夏医学院2006级研究生,宁夏银川750004

出　　处：《中国医院药学杂志》2009年第20期1720-1724,共5页Chinese Journal of Hospital Pharmacy

基　　金：重庆市卫生局资助项目(编号:渝中医[2006]27号2006-B-36)

摘　　要：目的:观察柴葛口服液(II)对四氯化碳肝纤维化大鼠体内模型的作用。方法:采用皮下注射四氯化碳诱导Wistar大鼠肝纤维化动物模型后,每天灌胃给予PO柴葛口服液(II)0.4mL.kg-1.d-1至第12周,酶动力学法检测分析肝功能,放射免疫分析法测定透明质胶(HA)、III型前胶原(PCIII)、层黏连蛋白(LN)、IV型胶原(IVC)等血清肝纤维化标志物指标,SP免疫组化法检测肝组织中α-肌动蛋白(α-SMA)、转化生长因子β1(TGF-β1)表达的影响,用苏木精-伊红染色(HE)和胶原纤维染色观察大鼠肝脏病理变化。结果:10周后,治疗组与模型组ALT、AST、MAO及HA、PC III、LN、IV-C等指标相比差异有显著性(P<0.05),并且治疗组肝组织纤维化程度分级较模型组逐渐好转,胶原纤维所占面积显著缩小;α-SMA和TGF-β1在治疗组和模型组肝组织中的表达差异也有显著性(P<0.01)。结论:柴葛口服液(II)能有效保护肝细胞,减轻肝纤维化。OBJECTIVE To study the effect of Chaige oral liquid（Ⅱ）in Wistar with hepatic fibrosis. METHODS The experimental model of hepatic fibrosis was induced by hypodermical injection of carbon tetrachloride （CCL） in Wistar rat,Chaige oral liquid（Ⅱ） was orally given for 10 weeks（0. 4 mL· kg^-1 body weight per day）, The samples were stained with hematoxylin and eosin for histopatbological examination. Masson staining was used to observe the liver fibrosis. Liver functions were measured by enzymatic kinetic analysis. The levels of α-smooth muscle aetin （α-SMA）and transforming growth factor-β1 （TGF-β1） expression in liver tissues were measured by SP immunohistochemistry method, The liver sign of hepatic fibrosis Hyaluronicacid （HA）, PrecollagentypeⅢ（PCⅢ）, laminin（LN） and Ⅳ collagen （IVC） were measured by Radioinununoassay（RIA） method. RESULTS After ten weeks of treatment, there were marked difference on ALT,AST,MAOand HA,PCⅢ,LN,Ⅳ C in treatment group than that in model group （P〈0. 05）. The levels of ccSMA and TGF -β1 expression were significantly different between treatment group and model group （P〈0. 01）. CONCLUSION The Chaige oral liquid（Ⅱ） can effectively protect hepatic cells, alleviate hepatic fibrosis.

关 键 词：大鼠 柴葛口服液(Ⅱ) 肝纤维化 四氯化碳 放射免疫分析法 免疫组织化学法 

分 类 号：R285.6[医药卫生—中药学]