加味六味地黄汤对环磷酰胺致幼年大鼠肝损害的保护作用  

Study on the protective effect of Jiawei Liuwei Dihuang recipe on the hepatic injury of the infancy rats induced by cyclophosphamide

作　　者：姜荣燕[1] 朱立平[1] 陈述英[1] 梁秀玲[1] 李然然[1] 

机构地区：[1]泰山医学院附属医院儿科,山东泰安271000

出　　处：《中国中西医结合儿科学》2009年第5期445-447,F0003,共4页Chinese Pediatrics of Integrated Traditional and Western Medicine

摘　　要：目的探讨加味六味地黄汤对环磷酰胺致幼年大鼠肝损害的保护作用。方法2周龄及3周龄Wistar大鼠各32只,每一周龄段按随机数字表法各分为3组,正常组8只,对照组12只和实验组12只。实验组灌胃加味六味地黄汤,2周龄大鼠咽饲量为每只每次0.6mL,3周龄咽饲量为每只每次0.9mL,每日2次,连续14d。对照组根据大鼠周龄喂饲等量生理盐水,每日2次,连续14d。实验组和对照组第7天和第8天腹腔注射环磷酰胺100mg/(kg.d);正常组常规喂养,第7天和第8天腹腔注射等量生理盐水。3组均于第15天检测血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)的水平。苏木精-伊红染色镜检观察肝组织病理学改变。结果2周龄及3周龄对照组大鼠ALT、AST均明显高于正常组,差异有统计学意义(P<0.05),且实验组ALT、AST均明显低于对照组,差异有统计学意义(P<0.05);光镜下对照组肝细胞广泛性的中度至重度水变性,实验组肝细胞水肿明显减轻。结论加味六味地黄汤对环磷酰胺致幼年大鼠肝损伤有一定的保护作用。Objective To explore the protective effect of Jiawei Liuwei Dihuang recipe on the hepatic injury of the young rats induced by cyclophosphamide（CP）. Methods A total of 32 2-week-old and 3-week- old Wistar rats were divided into three groups, each week-old segment according to the random number table, normal group 8, control group 12 and experimental group 12. Experimental group was fed Jiawei Liuwei Dihuang recipe, 2-week-old rats were fed pharyngeal volume 0. 6 mL for each, for 3 weeks of age,0. 9 mL,twice a day for 14 days. The control group rats were fed normal saline according to weeks, twice a day for 14 days. The experimental group and control group were intraperitoneally injected cyclophosphamide 100 mg/（kg · d） on the 7th day and 8th day; with normal routine feeding, equivalent normal saline was intraperitoneally injected on the 7th day and 8th day for the normal group. Serum alanine aminotransferase（ALT） and aspartate aminotransferase（AST） were detected on the 15th day. After hematoxylin-eosin staining, the pathological changes in liver tissue were observed by microscope. Results The ALT and AST of the 2-week-old and 3-week- old control rats were significantly higher than the normal group, the difference being of statistical significance（P〈0.05）; ALT and AST of the experimental group were significantly lower than the control group, the difference being of statistical significance（P〈0.05）. Under light microscope, the cellular swelling of the hepatocyte was moderate to severe in the control group, while liver cell edema was significantly relieved in the experimental group. Conclusion Jiawei Liuwei Dihuang recipe can protect the young rats from the hepatic injury induced by cyclophosphamide.

关 键 词：加味六味地黄汤/治疗应用 环磷酰胺/中毒 肝/损伤 大鼠 Wistar 动物 实验 

分 类 号：R285.5[医药卫生—中药学]