ELISA试验加样误差对试验结果的影响分析  

Influence of sampling errors on ELISA test results

作　　者：袁红[1] 毛旖[2] 黄文芳[1] 

机构地区：[1]四川省人民医院检验科,成都610072 [2]重庆医科大学医学检验系,400016

出　　处：《国际检验医学杂志》2009年第9期835-837,共3页International Journal of Laboratory Medicine

基　　金：四川省重点攻关课题(303005002106007)

摘　　要：目的评价不同程度加样误差对ELISA试验结果的影响。方法分别在标准加样量基础上递减1、2、3、4μL和递增1、2、3μL进行加样,比较加样差异对HBsAg、HCV和TP ELISA检测结果的影响。结果随加样量的增加,S/CO值呈上升趋势,HBsAg和TP(除+3组)与对照组的平均S/CO)值比较,差异无统计学意义(P>0.05);各组S/CO值虽存在差异,但差异无统计学意义(P>0.05)。HCV的-2、-1、+1组S/CO值虽存在差异,但差异无统计学意义(P>0.05);而-4、-3组和+2、+3组与对照组相比,差异有统计学意义(P<0.05)。各试验组平均阳性率差(与对照组相减的绝对值)随标准加样量的减少呈增大趋势,HBsAg、HCV、TP加样量不足和加样过量的试验组之间的平均阳性率差相比较.差异有统计学意义(P<0.05)。结论加样误差对ELISA试验S/CO值和阳性率造成不同程度的影响,其影响程度随标准加样量的减少呈增大趋势。Objective To evaluate the effect of various sampling errors on ELISA test results. Methods Standard sample volume,standard sample volume reducing 1,2,3,4 μL or adding 1, 2,3μL were respectively pipetted into the wells of a microplate, follwed by routine operation of ELISA test. Then the influence of various sampling errors was analyzed on ELISA test results of HBsAg, HCV and TP. Results S/CO value was increased with the increase of sample volume. The statistical difference of mean S/CO value of HBsAg and TP was only found between sample volume adding 3μL group and control group（P〈0.05）. For HCV result, there were significant differences between standard sample volume adding 2,3μL or reducing 3,4μL groups and control group（P〈0. 05）, while no obvious differences were found in the other groups（P〉0.05）. The difference of mean positive rate between experimental groups and control group showed an increasing tendency with the reduction of sample volume,and significant differences in HBsAg, HCV and TP results were also found between sample volume increase groups and reduction groups（P〈0.05）. Conclusion Various sampling error＇s influence ELISA test results to different degrees,and the extent increases with the reduction of standard sample volume.

关 键 词：酶联免疫吸附测定 质量控制 校准 

分 类 号：R446.61[医药卫生—诊断学]