绿玉菊离体培养与快速繁殖  

Rapid Propagation and in Vitro Culture of Senecio macroglossus

作　　者：孙成江[1] 林萍[2] 熊桂华[2] 陈梅[2] 何铃[1] 翟丽丽[2] 

机构地区：[1]文山学院,文山663000 [2]西南林学院

出　　处：《东北林业大学学报》2009年第10期29-31,43,共4页Journal of Northeast Forestry University

基　　金：云南省科技计划援助项目(2008CA006)

摘　　要：用绿玉菊叶片、不带腋芽茎段、带腋芽茎段和茎尖为外植体进行离体培养研究,分析了影响愈伤组织形成的因素及分化困难的原因。研究结果表明:在愈伤组织诱导时,不带腋芽茎段较嫩叶易产生愈伤组织,是首选外植体;6-BA1.0mg·L-1+NAA0.05mg·L-1的激素配比对茎段愈伤组织诱导有利,诱导率高达100%;在MS基本培养基中,pH值为5.4较适宜愈伤组织的分化,且6-BA3.0mg·L-1+NAA0.01mg·L-1对愈伤组织分化不定芽的效果较好,分化不定芽均数达11.10个。茎尖及带腋芽茎段在MS+6-BA2.0mg·L-1+NAA0.01mg.L-1的培养基上,能直接诱导出大量生长健壮的丛生芽,生根在MS+NAA0.05mg·L-1的培养基上进行,生根率高达100%,根多而壮,植株长势好。The factors influencing callus formation and differentiation were studied by in vitro culture of different parts of organs, including leaf, stem without auxiliary bud, stem with auxiliary bud, and stem tip, using Senecio macroglossus as experimental materials. Results showed that the callus could be induced more easily by stem without auxiliary bud than by leaf in the culture medium of MS supplemented with 1.0 mg/L 6-BA and 0.05 mg/L NAA, and the callus induction rate could reach 100 percent. The best medium for callus differentiation was MS with 3.0 mg/L 6-BA and 0. 01 mg/L NAA at pH 5.4, and the auxiliary buds could reach 11.10. The cluster buds could be induced by stem tip and stem with auxiliary bud in the medium of MS with 2.0 mg/L 6-BA and 0.01 mg/L NAA. The optimal culture medium for rooting was MS with 0. 05 mg/L NAA, and the induction rate could reach 100 percent. The plants with sturdy roots grew well.

关 键 词：绿玉菊 离体培养 快速繁殖 

分 类 号：S5[农业科学—作物学]