高压氧对皮肤移植小鼠T淋巴细胞粘附分子表达的影响  

The effect of hyperbaric oxygen on expression of adhesion molecules of Tlymphocyte in skin transplanted mice

作　　者：于艳秋[1,2] 王常慧[1,2] 张海鹏[1,2] 陈学新[1,2] 郝鸣政[1,2] 

机构地区：[1]中国医科大学病理生理教研室 [2]中国医科大学第一临床学院高压氧科

出　　处：《中华理疗杂志》1998年第6期339-341,共3页Chinese Journal of Physical Therapy

基　　金：国家自然科学基金

摘　　要：目的：探讨高压氧对小鼠Ｔ淋巴细胞表面粘附分子表达的影响。方法：皮肤移植，供鼠ＢＡＬＢ／Ｃ小鼠，受鼠Ｃ５７ＢＬ／６小鼠，高压氧及高压氧和雷公藤多甙联合应用组小鼠每天用９９．２％氧，０．２５ＭＰａ作用１．５小时，联合应用组每天给雷公藤多甙２０ｍｇ／ｋｇ灌胃，对照组小鼠不做任何处理，移植后１４天取脾，分离淋巴细胞，用流式细胞仪法分别检测ＣＤ３＋、ＣＤ４＋、ＣＤ８＋、ＣＤ４＋ＣＤ１１ａ＋、ＣＤ４＋ＣＤ１８＋、ＣＤ８＋ＣＤ１１ａ＋、ＣＤ８＋ＣＤ１８＋表达情况。结果：各测试指标高压氧组，联合应用组均低于对照组，高压氧组低于联合应用组。结论：高压氧抑制Ｔ淋巴细胞表面粘附分子的表达，影响Ｔ淋巴细胞的活化与增殖。Objective:The paper was to study the effect of hyperbaric oxygen(HBO) on expression of adhesion molecules of T lymphocyte.Methods:The donor mice BALB/C and receptor mice C 57 BL/6 were tested for skin transplantation.These mice were randomly divided into HBO group,HBO plus Glycosides Tripterygium Wilfordii hook(GTW) group and control group.The experinmental mice were treated with 99.2% oxygen under 0.25MPa for 1.5hrs or oral GTW(20mg/kg).The control group was without any treatment.After14 days,the spleens were extracted for separating lymphocytes, the conditions of expression of CD 3 +、CD 4 +、CD 8 +、CD 4 +CD 11a +、CD 4 +CD 18 +、CD 8 +CD 11a +、CD 8 +CD 18 + were detected with flow cytometry. Results:All indexes of HBO group,HBO plus GTW group are lower than those of control group.And all indexes of HBO group are the lowest.Conclusion:HBO has inhibiting effect on expression of adhesion molecules of T lymphocyte,affecting activation and multiplication of T-lymphocyte,and inhibiting immune rejection.

关 键 词：高压氧 皮肤移植 T细胞粘附分子 

分 类 号：R622.1[医药卫生—整形外科] R459.6[医药卫生—外科学]