棉花GhGAI1基因克隆及其功能的初步分析  

Cloning and Preliminary Functional Analysis of GhGAI1 Gene from Gossypium hirsutum L

作　　者：王拴锁[1] 曲廷云[1] 薛金教[1] 李晓明[1] 顾冉冉[1] 郑银英[1] 崔百明[1] 

机构地区：[1]石河子大学农业生物技术重点实验室/石河子大学生命科学学院,石河子832003

出　　处：《石河子大学学报（自然科学版）》2009年第5期548-553,共6页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金(307600991);新疆兵团博士基金项目(2007JC08)

摘　　要：DELLA蛋白是赤霉素信号通路中重要的蛋白质作用因子,负调节植物体中赤霉素的水平,同时受到生长素、脱落酸和乙烯的共同调节。根据已知的生物信息数据,本研究克隆了棉花GhGAI1基因,序列分析表明它编码DELLA蛋白。构建GFP与GhGAI1融合蛋白重组质粒,利用根癌农杆菌介导的花浸泡转基因方法转化拟南芥co-lumbia生态型植株,进行过量表达实验。结果表明,GFP-GhGAI1融合蛋白定位于细胞核并且在施加赤霉素后迅速降解,表明该基因编码蛋白参与赤霉素信号通路。As a kind of regulator in GA signaling, DELLA not only negtively regulates GA lever in plant, but also plays a role in Auxin,Abscisic Acid,ethylene signaling. We have cloned GhGAl1 gene which encodes DELLA protein in cotton with the reseve genetic methods according to bioinformation knowledge. The GFP and GhGAl1 fusion protein recombinant were constructed and transformed into arabidopsis ecotype columbia by floral-dipping method. The result shows that GFP-GhGAl1 fusion protein localized to the nucleus and degraded rapidly after GA treating. These result suggested that the protein which GhGAl1 encoded is an important regulator of GA signal.

关 键 词：陆地棉 拟南芥 DELLA蛋白 赤霉素 

分 类 号：S561[农业科学—作物学] Q751[生物学—分子生物学]