3,4-二羟基苯乙酮对缺氧所致猪肺动脉平滑肌细胞增殖的影响  

The Effect of 3,4-dihydroxyacetophenone on Hypoxia-induced Proliferation of the Smooth Muscle Cells in Porcine Pulmonary Artery

作　　者：郝天玲[1] 郝春荣[1] 王迪浔[1] 

机构地区：[1]同济医科大学卫生部呼吸系疾病重点实验室,武汉430030

出　　处：《同济医科大学学报》1998年第6期431-433,共3页Acta Universitatis Medicinae Tongji

基　　金：国家八五攻关项目(No.85-915-03-06)

摘　　要：用结晶紫染色法＾３Ｈ－ＴｄＲ掺入，增殖细胞核抗原免疫化学法检测３，４－二羟基苯乙酮对缺氧所致独肺动脉平滑肌增殖的影响。结果发现缺氧条件下培养的ＰＡＳＭＣ用以上３种方法测得结果分别为常氧组的。The effect of 3, 4-dihydroxyacetophcnone (DHAP) on the proliferation of the cultured pulmonary arterial smooth muscle cells (PASMC) induced by hypoxia was studied with methods of crystal violet staining. 3H-TdR incorporation and proliferating cell nuclear antigen (PCNA) immunocytochemistry. The results showed that the data detected by these three methods in the hypoxic PASMC group were (117. 1±4. 7) %, (131.2±17.0) %, (112.8±6. 2) % respectively, as much as those in the normoxic group (P<0.01 for all), which implied that hypoxia 3,4-PHAP could inhibit the proliferation of PASMC. induced by hypoxia in a dose-dependent manner. Since the hyperplasia of SMC contributes to the remodeling of pulmonary vessels in chronic hypoxic pulmonary hypertension, 3,4-DHAP might be of benefit in its prevention and treatment.

关 键 词：二羟基苯乙酮 缺氧 细胞增殖 肺动脉高压 PASMC 

分 类 号：R543.2[医药卫生—心血管疾病] R972[医药卫生—内科学]