切应力对培养人脐静脉内皮细胞摄取脂质的影响  

EFFECT OF SHEAR STRESS ON PINOCYTOSIS OF LOW DENSITY LIPOPROTEIN BY CULTURING HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS

作　　者：王克强[1] 贾剑国 江国伟[1] 艾珣 赵子琴[2] 张庆蕴 

机构地区：[1]上海医科大学解剖学教研室,上海200040 [2]上海医科大学法医教研室,上海200032 [3]上海药剂学校,上海200042

出　　处：《解剖学杂志》1998年第6期512-515,共4页Chinese Journal of Anatomy

摘　　要：目的:了解切应力对血管内皮细胞脂质摄取的影响.方法:利用可模拟生理及超生理范围脉动切应力的循环装置,分别模拟19.29,56.25.385,71 dynes/cm^2脉动切应力作用培养人脐静脉内皮细胞,免疫组化定量分析内皮细胞对低密度脂蛋白吞饮的影响.结果:脉动切应力可以促进内皮细胞对低密度脂蛋白的吞饮,并且其促进程度与切应力大小呈正相关.说明体内血流切应力在血管动脉粥样硬化病灶形成的过程中具有极其重要的作用.To study the effect of shear stress on the pinocytosis of low density lipoprotein by vascular endothelial cells. Methods: The parallel plate flow-loading apparatus, which can stimu-late physiological and superphysiological pulsatile shear stress, and immunocytochemical methods was applied to quantitate the stained particles of 1DL in endothelial cells. Results: The increase in LDL endocytosis of endothelial cells was dependent on the increase in pulsatile shear stress. Conclusion: Shear stress may play an important role in the localization and development of atherosclerosis lesions.

关 键 词：脐静脉 内皮细胞 动脉粥样硬化 切应力 培养 

分 类 号：R543.502[医药卫生—心血管疾病] R331.1[医药卫生—内科学]