IL-17对日本血吸虫感染抗体及Th应答的影响  

Studies on impact of IL-17 on antibody and Th responses in mice induced by Schistosoma japonicum infection

作　　者：温小云[1] 迟莹[1] 贺蕾[1] 周莎[1] 朱继峰[1] 张萃[1] 刘丰[1] 苏川[1] 

机构地区：[1]南京医科大学病原生物学系,南京210029

出　　处：《中国血吸虫病防治杂志》2009年第5期373-377,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(30872206;30571629)

摘　　要：目的观察IL-17在日本血吸虫感染小鼠中对抗体及Th细胞免疫应答的影响。方法在小鼠日本血吸虫感染前后,连续多次腹腔注射rm-IL-17细胞因子(实验组1)或PBS(对照组1),连续多次腹腔注射anti-IL-17中和抗体(实验组2)或同型抗体(对照组2)。运用流式细胞术,采用表面分子、胞内因子同时染色的三色标记法,对日本血吸虫感染小鼠脾脏、淋巴结中的Th17、Th1和Th2细胞亚群占CD4+T细胞的比例进行观察;同时采用ELISA法检测各组小鼠血清中血吸虫抗原特异性总IgG及亚类IgG1和IgG2a的水平。结果实验组1 CD4+T细胞中的Th17、Th1和Th2细胞亚群的比例分别是对照组1的1.09倍(P(0.05)、1.17倍(P(0.05)和1.15倍(P(0.05);实验组2 CD4+T细胞中的Th17、Th1和Th2细胞亚群的比例分别是对照组2的1.01倍(P(0.05)、1.11倍(P(0.05)和0.98倍(P(0.05)。rm-IL-17注射后仅可溶性虫卵抗原(SEA)特异性IgG1水平显著下降(P(0.05)、可溶性成虫抗原(SWA)特异性IgG2a的水平升高(P(0.05),其他抗体水平均无显著变化;anti-IL-17中和抗体注射后,SEA特异性总IgG(P(0.05)、IgG2a(P(0.01)和SWA特异性IgG2a(P(0.01)水平显著升高,其他抗体水平均无显著变化。结论IL-17可能不会显著增强或降低宿主的血吸虫特异性Th细胞应答,而可能对IgG类抗体的产生具有某种程度的抑制作用。Objective To observe the antibody and Th responses affected by IL-17 in mice infected with Schistosomejaponicum. Methods By repeated administration of rmIL-17 （ test group 1 ） or PBS （ control group 1 ）, or anti-IL-17 neutralizing mAb （ test group 2 ） or its IgG2a isotype control antibody （ control group 2 ） before and after Schistosoma japonicum infection, the proportions of Thl7, Thl and Th2 populations in C57BL/6 mouse spleen and lymph nodes were stained with surface molecule and intracellular cytokines and examined by three-color flow cytometery. Meanwhile, the levels of schistosome antigen specific IgG, IgG1 and IgG2a antibodies in mouse sera were detected by ELISA. Results In the rmIL-17 injected group, the proportions of Th17,Thl and Th2 populations in CD4^＋T cells were 1.09 folds （P〉0.05） , 1.17 folds （P 〉 0.05） and 1.15 folds （P 〉 0.05 ） of those in PBS injected control group, respectively. In the anti-IL-17 neutralizing mAb injected group, the proportions of Thl7,Thl and Th2 populations in CD4＊T cells were 1.01 folds （P 〉 0.05）, 1.11 folds （P 〉 0.05） and 0.98 folds （P 〉 0.05 ） of those in the IgG2a isotype antibody injected control group, respectively. In the rmIL-17 injected group, SEA spcific IgGl significantly decreased （ P 〈 0.05 ） and SWA specific IgG2a slightly increased （ P 〈 0.05 ）, while the other antibodies did not change significantly. In the anti-IL-17 neutralizing mAb injected group, SEA specific IgG （P 〈 0.05 ）, IgG2a （P 〈 0.01 ） and SWA specific IgG2a （P 〈 0.01 ） significantly increased,but the other antibodies did not change significantly. Conclusion IL-17 might not ＂affect the Th response but down-regulate the production of antibodies during Schistosoma japonicum infection, and the related mechanism needs further illustration.

关 键 词：日本血吸虫 IL-17 TH细胞 抗体 

分 类 号：R383.24[医药卫生—医学寄生虫学]