邻菲罗啉化学发光体系测定羟自由基的建立  

The Establishment of o phenathroline Chemiluminescent System for Measuring ·OH Radical

作　　者：范小兵[1] 李慈娟[1] 沙大年[1] 胡卫乐[1] 胡天喜[1] 

机构地区：[1]上海交通大学昂立生物医药研究所

出　　处：《基础医学与临床》1998年第6期68-71,共4页Basic and Clinical Medicine

摘　　要：本文描述了一个测量羟自由基（ＯＨ）的化学体系。该体系由１０×１０３ｍｏｌ／Ｌ邻菲罗啉溶液５０～１００μｌ，１０×１０３ｍｏｌ／Ｌ抗坏血酸溶液２０μｌ，１０×１０３ｍｏｌ／Ｌ硫酸铜溶液５０μｌ，硼砂硼酸缓冲液所组成，由４４×１０５ｍｏｌ／ＬＨ２Ｏ２溶液５０～１００μｌ启动发光，反应总体积为１ｍｌ，反应发光强度强，稳定时间可达１ｍｉｎ以上，经对ＯＨ的清除剂硫脲试验，线性范围为５０×１０６ｍｏｌ／Ｌ～５０×１０４ｍｏｌ／Ｌ，最低可检测限为５０×１０７ｍｏｌ／Ｌ，该体系有良好的稳定性，批内变异系数为０４％（ｎ＝８），批间变异系数为０５％（ｎ＝１３）。In this paper,a chemiluminescent system for measuring ·OH radical was described. It is composed of 50～100μl 1 0×10 3 mol/L o phenathroline solution,20 μl 1 0×10 3 mol/L ascorbate solution,50μl 1 0×10 3 mol/L CuSO 4 solution and borax boracic acid buffer solution. 50～100μl 4 4×10 5 mol/L H 2O 2 solution was used to start on the chemiluminescent. The volume of reaction was 1ml. The luminous intensity of reaction is very strong. The stabilizing time is more than 1 min. The ·OH radical scavengering test by thiourea showed that the linear rang was 5 0×10 6 mol/L 5 0×10 4 mol/L,and the minimum limit was 5 0×10 7 mol/L . This system is very steady. The intrabatch variation coefficient and the interbatch variation coefficient was 0 4%(n=8)and 0 5% (n=13),respectively.

关 键 词：邻菲罗啉 化学发光体系 羟自由基 测定 

分 类 号：O621.146[理学—有机化学] O657.3[理学—化学]