PCR-ELISA法及荧光定量PCR法检测HBV感染者精液HBV-DNA的探讨  

Testing HBV-DNA in semen with PCR-ELISA and FQ-PCR

作　　者：钟剑峰[1] 高兴成[1] 黄伟佳[1] 史利华[1] 

机构地区：[1]广州医学院第三附属医院,广东广州510150

出　　处：《海南医学》2009年第11期9-11,22,共4页Hainan Medical Journal

基　　金：广州市医药卫生科技项目(编号:2007-YB-174)

摘　　要：目的探讨PCR-ELISA法及荧光定量PCR法(FQ-PCR)检测HBV感染者精液中HBV-DNA检出情况及其临床意义。方法选取男性HBV感染者60例,采集患者精液、静脉血,用PCR-ELISA法来检测患者精液及血清中HBV-DNA。同时采用荧光定量PCR检测患者精液中HBV-DNA。结果PCR-ELISA法:60例精液标本HBVDNA阳性12例,阳性率18.33%,拷贝数为1.267×103-6.532×105/ml,平均拷贝数为4.781×104/ml;60例血清标本阳性53例,阳性率为88.33%,拷贝数1.462×103-5.153×107/ml,平均拷贝数为2.451×106/ml。荧光定量PCR法:60例精液标本HBVDNA阳性13例,阳性率21.67%,拷贝数为1.357×103-7.113×105/ml,平均拷贝数为4.983×104/ml;两种检验方法检测精液HBV-DNA的阳性检出率比较无显著性意义(P>0.05)。结论PCR-ELISA法与荧光定量PCR定量检测精液中HBVDNA同样具有较强的特异性和灵敏度,为临床了解乙型肝炎患者精液中肝炎病毒的感染状态提供了帮助,具有重要的临床意义。Objective To evaluate the value of PCR - ELISA and FQ - PCR to test HBV DNA in HBV sufferers＇semens. Methods HBV DNA in 60 HBV sufferers＇ semens and serums were tested with quantitative PCR - ELISA and FQ - PCR. Results Using PCR - ELISA, there were 12 positive samples of HBV DNA in 60 semen samples, and the positive was 18.33%. The copies were 1. 267 ×10^3 - 6.532 ×10^5/ml, and the average copy was 4.781 ×10^4/ml. There were 53 positive samples of HBV DNA in 60 serums samples, and the positive is 88.33%. The copies were 1.462 ×10^5 -5. 153 ×10^7/ml, and the average copy is 2.451 ×10^6/ml. Using FQ - PCR, there were 13 positive samples of HBV DNA in 60 semen samples, and the positive was 21.67%. The copies were 1. 357 ×10^3 -7. 113 ×10^5/ml, and the average copy was 4. 983 ×10^4/ml. There are no difference between PCR- ELISA and FQ- PCR. Conclusion PCR -ELISA quantitative test and FQ -PCR have similar speciality and sensitivity, which has important clinical significance to evaluate the situation of HBV DNA viruses in semen samples.

关 键 词：PCR-ELISA FQ-PCR乙型肝炎病毒DNA 精液 

分 类 号：R446.6[医药卫生—诊断学]