壳聚糖对羟基自由基的清除作用研究  

A Research on Scavenging Activity to Hydroxyl Radicals by Chitosan

作　　者：吴飞鹏[1] 龚超群[1] 蔡继业[1] 马淑媛[2] 王小平[2] 

机构地区：[1]暨南大学化学系,广东广州510632 [2]暨南大学附属第一医院,广东广州510630

出　　处：《激光生物学报》2009年第5期609-613,646,共6页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(60578025);国家自然科学基金重点项目(30230350)

摘　　要：通过Fenton体系模拟了体内羟基自由基,并借助原子力显微镜(AFM)和通过荧光实验分别定性和定量研究了壳聚糖(CS)对羟基自由基的清除作用。通过AFM对红细胞成像来观察细胞的整体表面结构和微观结构,根据平均粗糙度和细胞微观结构的有序性来说明CS对羟基自由基的清除;由荧光实验中荧光强度的改变来说明不同浓度和不同分子量的CS清除自由基的效率。两种测量手段都一致地表明了CS能有效地清除自由基,而且清除效率随着CS浓度的增大而升高,随着CS分子量的增大而降低。Simulate hydroxyl radical in vivo by Fenton system and use AFM and fluorescence experiment to show the hydroxyl radical scavenging activities of chitosan qualitatively and quantitatively. AFM was used to observe the whole surface texture and microstructure of red blood cell, then the hydroxyl radical scavenging activity of chitosan is proved through the measurement of the average roughness and the order of microstructure; in the fluorescence experiment, the change of fluorescence intensity shows different scavenging activities of chitosan with varied concentration and molecular weight. The two methods drew a conclusion that chitosan can scavenge hydroxyl radical effectively, and increasing the concentration or reducing the molecular weight can give a more effective scavenging activity on hydroxyl radical.

关 键 词：原子力显微镜 红细胞 羟基自由基 

分 类 号：R318[医药卫生—生物医学工程]