缺氧处理小白鼠乳酸脱氢酶同工酶的辅酶化和去辅酶化调控作用  

Regulating Function of Enzymization and Deenzymization of the Lactate Dehydrogenase Isozymes in the Mouse Tissues during Hypoxia

作　　者：袁明秀[1] 邵宏兵 耿震 刘建 邓爱平[1] 金浩方[2] 

机构地区：[1]北京大学生命科学学院,北京100871 [2]北京大学技物系

出　　处：《中国医学科学院学报》1998年第6期449-453,共5页Acta Academiae Medicinae Sinicae

摘　　要：目的　 研究生物缺氧应激反应调控模式——小白鼠轻度缺氧心肌乳酸脱氢酶谱变化特点。方法　 以小白鼠心肌为材料 ,人工缺氧 ,乳酸脱氢酶酶活性特异染色。CS- 91 0型双波长薄层层析扫描仪定量测定 L DH(1～ 5) 相对百分含量。结果　 轻度缺氧处理后小白鼠各组织的 L DH同工酶相对百分酶活性向以 H基为主的同工酶( LDH1 、L DH2 )方向移动 ,L DH1 ( H4)、LDH2 ( H3M)的相对百分酶活性升高 ,而除心肌外组织的 LDH5( M4)相对百分酶活性显著降低 ,LDH4( HM3)与 L DH5的相对百分酶活性也有所下降。人工轻度缺氧处理的心肌样品 PAGE电泳后 ,在 L DH同工酶酶谱上有规律的产生活性亚带 ,其规律是 LDH1 ( 0条 ) ,L DH2 ( 0～ 1条 ) ,LDH3( 0～ 2条 ) ,LDH4( 1～ 3条 ) ,L DH5( 2～ 4条 ) ,缺氧处理后的小白鼠心肌样品中加入适量的 NAD+再走 PAGE电泳 ,L DH2 ～ L DH54种同工酶的活性酶谱上的活性亚带数减少或完全消失。结论　 乳酸脱氢酶辅酶化与去辅酶化负反馈调控模式是小白鼠轻度缺氧应激反应机制之一。Objective Study the characterestics of changes of L DH enzyme map of mouse under slight hypoxia.Methods Treated mouse with artificial hypoxia,various tissue biopsies were made for the test of L DH enzymatic activity by specific staining technical LDH(1 - 5) rela- tive percent enzymatic activity were measured with CS- 91 0 dual- wavelength thin- layer chro- matography scanner.Results The relative percent enzymatic activity of LDH isozymes of various tissues after slight hypoxia shift to the isozymes L DH1 and LDH2 ,whose principal subunits are H subunits,and the relative percent enzymatic activity of LDH1 ( H4) ,LDH2 - ( H3M) increased( the relative percent enzymatic activity of LDH2 increased markedly,P< 0 .0 5 ) ,while L DH ( M4) in various tissues decreased prominently except the cardiac muscle, and that of L DH4( HM3) decreased as well.After the PAGE of the hypoxia treated cardiac muscle specimen was made,activity subbands originated regularly in the isozyme pattern of L DH,with the regularity of L DH1 ( 0 subband) ,LDH2 ( 0～ 1 subbands) ,LDH3( 0～ 2 subbands) ,LDH4( 1～ 3subbands) ,L DH5( 2～ 4subbands) .After adding appropriate amount of NAD+to mouse cardiac muscle specimen with hypoxia,PAGE showed the numbers of subbands of four kinds of isozymes( LDH2 ～LDH5) decreased even totally disappeared in the isozyme pattern. Conclusions The negative feedback regulation of enzymization and deen- zymization of L DH isozymes is one of mouse stress responses to slight hypoxia.

关 键 词：乳酸脱氢酶 同工酶 辅酶化 去辅酶化 缺氧 

分 类 号：Q554.1[生物学—生物化学]