抗纤益心方对扩张型心肌病大鼠心肌Ⅰ、Ⅲ型胶原mRNA表达的影响  

Effect of Kangxian Yixin Extract on collagen typeⅠand Ⅲ of mRNA in rats with dilated cardiomyopathy

作　　者：王振涛[1] 韩丽华[1] 朱明军[2] 柴松波[1] 曹程浩[1] 

机构地区：[1]河南省中医院心血管病研究室,河南郑州450011 [2]河南中医学院第一附属医院心脏中心,河南郑州450003

出　　处：《中成药》2009年第10期1496-1500,共5页Chinese Traditional Patent Medicine

基　　金：河南省高校新世纪优秀人才支持计划(2005HANCET-07)

摘　　要：目的:探讨抗纤益心方(党参、黄芪、茯苓、白术、丹参、川芎、红花、赤芍、泽兰、益母草)抑制扩张型心肌病(DCM)大鼠心室重构的机制。方法:采用自主饮用呋喃唑酮水溶液建立DCM大鼠模型,8周后,存活大鼠随机分成5组(每组14只)16周时,处死动物。用免疫组化法(SP法)和逆转录-聚合酶链反应法(RT-PCR)检测心肌组织Ⅰ、Ⅲ型胶原的表达。结果:模型组心肌间质内Ⅰ、Ⅲ型胶原大量增生,与正常组比较有显著差异性(P<0.05);中西药联用组、大剂量组心肌内Ⅰ、Ⅲ型胶原较模型组明显减少(P<0.05);大剂量组与卡托普利组比较无差别(P>0.05),与小剂量组比较有统计学意义(P<0.05)。结论:DCM大鼠心肌呈明显纤维化,与Ⅰ、Ⅲ型胶原在心肌组织内较高的表达存在密切关系。抗纤益心方能够抑制心肌间质Ⅰ、Ⅲ型胶原蛋白的表达,从而起到抑制心室重构的作用。AIM： To explore the mechanisms of Kangxian Yixin Extract（ Radix Codonopsis, Radix Astragali Poria, Rhizoma Atractylodis macrocephalae, Radix et Rhizoma Salviae miltiorrhizae, Rhizoma Chuanxiong, Flos Carthami, Radix Paeoniae rubra, Herba Lycopi, Herba I_eonuri） on ventricular remodeling after dilated cardiomy- opathy. METHODS： Ventricular remodeling model was induced by giving Furazolidone in wistar rats for 8 weeks and survival rats were divided into 5 groups（ 14 rats each group） after another 8 weeks, all the rats were killed. The effects of Kangxian Yixin Extract on type Ⅰ and type Ⅲ collagen gene expression were determined with SP and RT-PCR methods. RESULTS ： Collagen Type Ⅰ and Ⅲ of model group was significantly higher than that of normal group （ P 〈 0.05 ） ; Type Ⅰ and Ⅲ level of mRNA in the the high dosage and captopril group and the high dosage of Kangxian Yixin group decreased significantly as compared with the model group （ P 〈 0.05 ） ; There was no differ ence in Collagen Type Ⅰ and Ⅲ of higher than that of the low dosage the high dosage group compared of group （ P 〈 0.05 ）. CONCLUSION the captopril group （ P 〉 0.05 ）, while ： Collagen Type Ⅰ and Ⅲ its mRNA expression are increased in rats with dilated cardiomyopathy. Effect of Kangxian Yixin may be related to resisting type Ⅰ and type Ⅲ collagen gene expression to inhibit the Extract on myocardial fibrosis rat＇s ventricular remodeling.

关 键 词：抗纤益心方 扩张型心肌病 心室重构 Ⅰ、Ⅲ型胶原 免疫组化法 逆转录-聚合酶链反应 

分 类 号：R285.6[医药卫生—中药学]