大豆疫霉根腐病子叶接种法抗病性鉴定  

Screening on Soybean Resistance to Phytophthora sojae using Cotyledon Inoculation Method

作　　者：于安亮[1] 徐鹏飞[1] 陈晨[1] 王金生[1] 吴俊江[2] 李宁辉[1] 李文滨[1] 马凤鸣[3] 邱丽娟[4] 常汝镇[4] 陈维元[5] 张淑珍[1] 

机构地区：[1]东北农业大学大豆研究所,教育部大豆生物学重点实验室,黑龙江哈尔滨150030 [2]黑龙江省农业科学院大豆研究所,黑龙江哈尔滨150086 [3]东北农业大学农学院,黑龙江哈尔滨150030 [4]中国农业科学院作物科学研究所,北京100081 [5]黑龙江省农业科学院绥化分院,黑龙江绥化152052

出　　处：《大豆科学》2009年第5期879-882,888,共5页Soybean Science

基　　金：国家自然科学基金资助项目(30671317,30810103063,30400285);中国博士后基金资助项目(20060400835);黑龙江省自然科学基金资助项目(C200814);国家重点基础研究发展计划资助项目(2004CB117203-4);黑龙江省新世纪人才培养计划资助项目(NCET-06-007);黑龙江省农科院博士后基金资助项目(LRB06-010);农业部寒地作物重点开放实验室开放课题资助项目

摘　　要：用子叶接种法鉴定了来自黑龙江、内蒙古、湖北、以及四川的65个大豆品种对疫霉根腐病1号生理小种的抗感情况。其中抗病品种11个,中间类型5个,感病品种49个。其鉴定结果与下胚轴接种法比较相同的有51个品种。经过DPS v7.05分析下胚轴接种法与子叶接种法之间的相关呈极显著,经r×c独立性测验得到χ2=1.46<χ0.05,22=5.99,2种方法差异不显著。证明了子叶接种法进行大豆疫霉根腐病抗性鉴定同样准确可行。此外还可以解决遗传分析上需要保存感病植株后代的问题,为遗传分析时鉴定疫霉根腐病抗感情况提供了可靠的方法。Soybean production is seriously affected by Phytophthora sojae, one kind of destructive fungal diseases. The most effective measure to prevent and control the diseases is to carry out disease-resistant breeding. In this paper,65 soybean varieties from Heilongjiang,Inner Mongolia,Hunan and Sichuan were evaluated the reactions to P. sojae race 1 by way of cotyledon inoculation. Of all the varieties tested, 11 were resistant ,49 were susceptible, and the rest were intermediate. Fifty-one varieties had the same reaction as those evaluated with hypocotyl inoculation method. There was significant correlation between cotyledon inoculation and hypocotyl inoculation method according to DPS V7.05 analysis. By the independent tests of r x c table of cotyledon inoculation and vaccination hypocotyl, there were no significant differences between the two methods. Cotyledon inoculation was proved to be feasible to evaluate soybean germplasm infection with P. sojae compared with the commonly used hypocotyl inoculation method. It not only provided a method to identify the soybean resistance to P. sojae, but also solved the problem that the seeds of susceptible plants generated from crossing progeny could not be kept using hypocotyl inoculation method.

关 键 词：抗病性鉴定 子叶接种法 下胚轴接种法 大豆疫霉根腐病 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]