肾上腺素诱导兔血小板聚集的实践与理论探讨  

The practice and theoretical exploration on rabbit platelet aggregation induced by adrenaline

作　　者：储钟禄[1,2] 宋春桥[1,2] 张桐芳[1,2] 陈育贵 黄才国[1,2] 

机构地区：[1]第二军医大学生化教研室 [2]第二军医大学附属长征医院血库

出　　处：《中国药理学通报》1998年第6期509-512,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金

摘　　要：目的创建以Ａｄｒ诱导兔血小板聚集的方法，并对受体分子特性作初步探讨。方法以高Ｋ＋缓冲液等取代兔ＰＲＰ中血浆，以Ａｄｒ诱导聚集，以Ａｐｙｒ证实结果。结果兔血小板悬浮于高Ｋ＋缓冲液时Ａｄｒ能单独诱导真正的聚集。结论由此推测血小板膜上α２肾上腺素受体分子可能为由两种亚单位组成：促聚亚单位和辅助亚单位。人辅助亚单位当Ａｄｒ浓度高达聚集阈值以上时可被激活，与促聚亚单位结合为活性的二聚体，与Ａｄｒ进一步结合发生聚集作用。AIM To create a method to induce rabbit platelet aggregation by adrenaline , and to explore theoretically the characteristics of the receptor molecule . METHOD To replace the blood[KG*4/5] plasma in rabbit PRP with high K + buffer , etc . for inducing platelet aggregation by adrenaline and adding apyrase to verify the results. RESULTS When rabbit platelets were suspended in high K + buffer the platelet aggregation was really induced by adrenaline alone. CONCLUSION It is supposed that α 2 adrenoceptor molecule on platelet membrane might contain two subunits

关 键 词：肾上腺素 血小板聚集 诱导 肾上腺素受体 

分 类 号：R977.11[医药卫生—药品] R331.143[医药卫生—药学]