褪黑素对类风湿关节炎病人外周血淋巴细胞增殖反应的影响及其G蛋白-cAMP信号转导机制的研究  

Effects of melatonin on peripheral blood lymphocyte proliferation from patients with rheumatoid arthritis and G protein coupled cAMP signal transduction

作　　者：魏伟[1,2] 徐星铭[1,2] 沈玉先[1,2] 丁长海 张安平[1,2] 徐叔云 

机构地区：[1]安徽医科大学临床药理研究所 [2]安徽医科大学附属医院肾内科

出　　处：《中国药理学通报》1998年第6期530-532,共3页Chinese Pharmacological Bulletin

基　　金：安徽省自然科学基金

摘　　要：目的研究褪黑素（ＭＴ）对类风湿关节炎（ＲＡ）病人外周血淋巴细胞（ＰＢＬ）增殖反应的影响及其Ｇ蛋白ｃＡＭＰ信号转导机制。方法淋巴细胞增殖法、ｃＡＭＰ放免测定及Ｇ蛋白功能分析。结果ＲＡ病人ＰＢＬ增殖能力低于正常人１０倍左右，ＭＴ在１×１０－８～１×１０－６ｍｏｌ·Ｌ－１能明显促进ＲＡ病人ＰＢＬ的增殖反应；ＲＡ病人ＰＢＬ的ｃＡＭＰ水平明显高于正常人，ＭＴ（１×１０－６ｍｏｌ·Ｌ－１）可部分使其降低，且ＭＴ的这一作用能被百日咳毒素（ＰＴ）取消。结论ＭＴ可促进ＲＡ病人ＰＢＬ的增殖反应。AIM To observe the effects of melatonin (MT) on peripheral blood lymphocyte (PBL) proliferation from patients with rheumatoid arthritis (RA) and G protein cAMP signal transduction. METHODS Lymphocyte proliferation, cAMP radioimmunoassay, etc. were used. RESULTS The PBL proliferation in RA patients was ten times lower than that in normal humans. MT (1×10 -8 ～1×10 -6 mol·L -1 ) significantly increased the PBL proliferation of RA patients. The cAMP levels of RA patients PBL was higher than that of normal human PBL, MT (1×10 -6 mol·L -1 ) could partially decrease the cAMP levels which can be abolished by pertussis toxin (PT). CONCLUSIONS MT can increase the proliferation of RA patients PBL. PT Senstitive Gi protein cAMP signal transduction may be one of important mechnisms of MT in regulationg immune responses.

关 键 词：褪黑素 类风湿关节炎 PBL G蛋白 CAMP 

分 类 号：R977.1[医药卫生—药品] R593.22[医药卫生—药学]