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作 者:骞爱荣[1] 胡丽芳[1] 狄升蒙[1] 张维[1] 高翔[1] 商澎[1]
机构地区:[1]西北工业大学生命科学院空间生物实验模拟技术国防重点学科实验室特殊环境生物物理学研究所,中国陕西西安710072
出 处:《生命科学研究》2009年第5期382-387,共6页Life Science Research
基 金:国家自然科学基金资助项目(30840030;30970706)
摘 要:采用激光共聚焦显微术研究微管微丝交联因子(MACF1)与成骨样细胞(MG-63及MC3T3)微丝/微管骨架、黏着斑之间的相互关系.结果表明,MACF1不连续地分布于微管纤维上,与微丝骨架部分共定位于胞质中,在很多的成骨细胞中可见MACF1分布于骨架相关的粘着斑处;细胞松弛素B影响了MACF1在成骨细胞中的分布,并有使其向细胞核周围及核内转位的趋势,秋水仙素对MACF1的分布无明显的影响.转染了siRNA-MACF1的MG-63细胞微丝骨架纤维分布不连续、微管骨架纤维分布紊乱.这些结果提示MACF1不仅起交联微丝及微管细胞骨架的作用,而且还可稳定细胞骨架;成骨细胞MACF1的分布更依赖于微丝骨架的完整性.The association of microtubule aetin crosslinking factorl (MACF1) with actin filaments (AFs), microtubule (MT) and focal adhesion were examined using laser scanning confocal microscopy (LSCM). Results showed that MACF1 aligned along MT filaments and partly co-localized with the AFs and MT in the Cytoplasm. In many osteoblast, anti-MACF1 labeling could be found in regions of focal adhesions. cytochalasin B caused translocation of MACF1 to the perinuclear region or nucleus, in contrast, colchicines had no obvious effects on the association of MACF1 with cytoskeleton. AFs were discrete and disrupted, and MTs were disorganized in MG-63 cells tranfeeted with siRNA-MACF1 compared with that in control cells. These results indicate that MACF1 plays an important role not only in linking cytoskeleton proteins but in stabilizing the cytoskeleton structure. The association of MACF1 with cytoskeleton primarily depends on the integrity of the actin network.
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