爱大霉素和庆大霉素对肾皮质线粒体^(45)Ca^(2+)摄取的影响  

The effects of etimicin and gentamicin on renal corticalmitochondria ^(45)Ca^(2+)uptake

作　　者：李忠东[1,2] 李培忠[1,2] 洪文清[1,2] 

机构地区：[1]空军总医院药理科 [2]军事医学科学院毒物药物研究所

出　　处：《中国药理学通报》1998年第3期258-259,共2页Chinese Pharmacological Bulletin

摘　　要：目的研究爱大霉素和庆大霉素对大鼠肾皮质线粒体钙摄取的影响。方法应用４５Ｃａ２＋示踪技术。结果两药在１ｍｍｏｌ·Ｌ－１时显著促进线粒体钙摄取（分别由每３０ｍｉｎ（１１３．５±７．５）μｍｏｌ·ｇ－１Ｐｒｏ上升到（１４８．０±２４．０）μｍｏｌ·ｇ－１Ｐｒｏ，（１１１．９±１３．０）μｍｏｌ·ｇ－１Ｐｒｏ上升到（１４０．１±１６．６）μｍｏｌ·ｇ－１Ｐｒｏ，Ｐ＜０．０５）；在１０μｍｏｌ·Ｌ－１时显著抑制线粒体钙摄取（分别由每３０ｍｉｎ（１１３．５±７．５）μｍｏｌ·ｇ－１Ｐｒｏ下降到（９２．３±５．７）μｍｏｌ·ｇ－１Ｐｒｏ，（１１１．９±１３．０）μｍｏｌ·ｇ－１Ｐｒｏ下降到（１４０．０±５．３）μｍｏｌ·ｇ－１Ｐｒｏ，Ｐ＜０．０５或Ｐ＜０．０１）。AIM To study the effects of etimicin and gentamicin on renal cortical mitochondria ^(45)Ca^(2+)uptake. METHODS Using ^(45)Ca^(2+) incorporation technique. RESULTS The two drugs at the concentration of 1 mmol·L-1 significantly promoted the ^(45)Ca^(2+)uptake 〔the former ascended from per 30 min(1135±75) to (1480±240) μmol·g-1 Pro, the latter from per 30 min(1119±130) to (1401±166) μmol·g-1 Pro, P<005〕, and at the concentration of 10 mmol·L-1, dramatically inhibited the mitochondria ^(45)Ca^(2+)uptake 〔the former descended from per 30 min(1135±75) to (923±57) μmol·g-1 Pro, the latter from per 30 min(1119±129) to (695±53) μmol·g-1 Pro, P<005 or P<001〕. CONCLUSION The two phase effects of the two drugs on mitochondria ^(45)Ca^(2+)uptake may correlate with their nephrotoxicity.

关 键 词：线粒体 钙摄取 爱大霉素 庆大霉素 抗生素 

分 类 号：R978.12[医药卫生—药品]