褪黑素对吗啡依赖小鼠的作用及其与脑啡肽关系的研究  

Effects of melatonin on morphineinduced mice dependence and relation with enkephalin

作　　者：魏伟[1] 沈玉先[1] 丁长海[1] 徐星铭[1] 刘泽源[1] 徐叔云[1] 

机构地区：[1]安徽医科大学临床药理研究所

出　　处：《中国药理学通报》1998年第1期72-74,共3页Chinese Pharmacological Bulletin

基　　金：安徽省自然科学研究基金

摘　　要：目的观察褪黑素（ＭＴ）对吗啡（Ｍｏｒ）致依赖性的作用及机制分析。方法连续ｉｐＭｏｒ与纳络酮催促建立小鼠依赖模型，甩尾法，淋巴细胞增殖反应和脑啡肽（Ｍｅｔ－Ｅｎｋ）放免测定。结果Ｍｏｒ依赖组小鼠痛阈下降，跳跃次数增加，肛温降低，伴有胸腺细胞增殖能力的低下，Ｍｏｒ依赖小鼠的脑和血浆Ｍｅｔ－Ｅｎｋ亦明显降低。本文首次发现ｉｇＭＴ（３０、９０ｍｇ·ｋｇ－１×７ｄ）能明显提高依赖性小鼠的痛阈，减少跳跃次数，恢复其肛温，阻止Ｍｏｒ对淋巴细胞功能的抑制，并能维持脑、血浆Ｍｅｔ－Ｅｎｋ接近正常对照水平。结论ＭＴ具有对抗Ｍｏｒ致小鼠依赖性作用，其机制与维持内源性Ｍｅｔ－Ｅｎｋ水平有关。AIM To observe the effects and mechanisms of melatonin (MT) on morphine (Mor)induced mice dependence. METHODS The Mordependentmice model was established by intraperitoneal (ip) administered Mor (10 mg·kg-1) for seven days and the pain threshold, thymocyte proliferation and methionineenkephalin(MetEnk) were determined by the tailflick, cell incubation and radioimmunoassay, respectively. RESULTS The pain threshold and the thymocytes proliferation in Mordependent mice were all decreased, and there were a marked increase in jumping number and a marked decrease in rectal temperature after naloxone (Nal) precipitation. The results also showed that the brain and plasma MetEnk levels were lower in Mordependent mice. MT(30,90 mg·kg-1,ig×7 d) could significantly antagonized the effects induced by Mor and maintained the brain and plasma MetEnk levels to normal status. CONCLUSIONS MT could antagonize the Morinduced mice dependence, which was related to its maintaining the levels of endogenous MetEnk.

关 键 词：褪黑素 吗啡依赖性 脑啡肽 

分 类 号：R977.1[医药卫生—药品] R595.5[医药卫生—药学]