血管紧张素Ⅱ快速诱导ApoE-/-小鼠腹主动脉夹层动脉瘤模型的建立及其MR成像  

Construction and MRI of angiotensin Ⅱ transiently induced abdominal aortic dissection in ApoE-/-mouse model

作　　者：赵瑞[1] 邓钢[1] 姚玉宇[1] 居胜红[1] 王中娟[1] 文颂[1] 陈骏[1] 金晖[1] 

机构地区：[1]东南大学附属中大医院放射科,江苏南京210009

出　　处：《中国医学影像技术》2009年第10期1749-1752,共4页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金重点项目(30830039);国家自然科学基金(30770264)

摘　　要：目的建立ApoE-/-小鼠腹主动脉夹层动脉瘤模型,探讨超高场强7.0TMR检测ApoE-/-小鼠腹主动脉瘤的价值。方法10月龄ApoE-/-小鼠饲以高脂饮食10周后,背部埋置血管紧张素Ⅱ缓释泵,AngⅡ分为1000ng/(kg·min)和500ng/(kg·min)组,对照组埋置生理盐水,装泵前及装泵后14天内行MR活体扫描,扫描后取腹主动脉行病理学检查。结果AngⅡ灌注高剂量组第6或第7天即形成夹层动脉瘤,T2WI示一侧管壁周可见新月形超高信号,病理证实为中膜与外膜间出血;低剂量组第13、14天后也见腹主动脉夹层动脉瘤形成,MR示管壁信号于T2WI及PDWI均明显增加,并可见斑点状高信号向腔外突出,与病理内膜断裂血流冲击中膜形成主动脉夹层一致。结论用2种剂量的血管紧张素Ⅱ灌注2周内均可成功建立ApoE-/-小鼠腹主动脉夹层动脉瘤模型,前组可在更短时间内建模成功。超高场强MR可成功用于活体检测腹主动脉夹层动脉瘤形成。Objective To establish abdominal aortic dissection model in ApoE-/-miee, and to evaluate the ability of 7.0T MR to detect the abdominal aortic artery aneurysms in ApoE-/-mice in vivo. Methods ApoE-/-mice aged 10 months were infused with angiotensin Ⅱ with 14 days Osmotic minipump after 10 weeks of high lipid diet. Two different doses of angiotensin Ⅱ were given to mice, i.e. 1000 ng/（kg · min） and 500 ng/（kg · min）, respectively. The contrast group was infused with saline water. The abdominal aortic artery was observed in vivo with MR before and within 14 days infusion. At last, the pathological changes of the abdominal artery were compared with MRI findings. Results After 6 or 7 days higher dose of angiotensin Ⅱ infusion, aortic dissection was seen. MR T2Wl showed crescent-shaped high signal in the vessel wall of one side,the pathological study identified the hematoma between media and adventitia. Abdominal aortic dissection aneurysms were also found in the mice 13 or 14 days after lower dose of angiotensin Ⅱ infusion, which were consistent with pathological studies. Besides, the signal of the vessel wall was significantly higher in both T2WI and PDWI sequences. There was excellent agreement between MR and histopathology. Conclusion Abdominal aortic dissection aneurysms model can be successfully established with different doses （1000 ng/（kg · min） and 500 ng/（kg · min）） of angiotensin 11 infusion into ApoE-/-mice fed with high lipid diet. High-resolution MR is able to visualize the abdominal aortic dissection aneurysm formation in vivo.

关 键 词：动脉瘤 腹 模型 动物 血管紧张素 磁共振成像 

分 类 号：R445.1[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]