雌激素改善卵巢切除合并慢性铝中毒对大鼠心脏Cx43表达和金属元素变化的影响  

Effect of estrogen replacement threapy on Cx43 expression and metal elements in ovariectomy and chronic aluminum toxication rat heart

作　　者：徐振平[1] 赵长安[2] 许娜[3] 张文[4] 郭志坤 

机构地区：[1]新乡医学院细胞生物及遗传学教研室 [2]新乡医学院生物化学与分子生物学教研室 [3]新乡医学院形态学实验室 [4]新乡医学院分析测试实验室 [5]河南省高等学校组织再生重点实验室,新乡453003

出　　处：《解剖学报》2009年第5期766-769,共4页Acta Anatomica Sinica

基　　金：河南省教育厅自然科学基金资助项目(20013100004;2008A330002)

摘　　要：目的探讨雌激素治疗卵巢切除合并慢性铝中毒大鼠,对心脏间隙连接蛋白43(Cx43)表达和金属离子含量的影响。方法40只6月龄雌性SD大鼠,随机分为卵巢假切除组(Sham组),卵巢切除组(OVX组)、卵巢切除合并慢性铝中毒组(OVX+Al组)和卵巢切除合并慢性铝中毒及尼尔雌醇灌胃组(OVX+Al+E2组)。于术后3个月,利用离子体发射光谱仪测定心脏金属元素含量,采用免疫组织化学和图像分析法观测心肌细胞Cx43表达。结果1.Cx43表达:Sham组心房肌Cx43遍布于心肌细胞侧面连接和端闰盘处。心室肌分布于心肌闰盘处。OVX组心房肌的Cx43颗粒部分由细胞侧-侧连接处移至闰盘处或杂乱排列,心室肌Cx43由闰盘处移至细胞侧-侧连接处或杂乱地无序排列,少部分出现在细胞质中,OVX+Al组Cx43排列类似于OVX组,且更加紊乱。OVX+Al+E2组心房肌Cx43回复正常分布,但心室肌未发现回复。2.图像分析结果,各组大鼠心肌细胞Cx43的面积构成比比较无显著差异。3.金属元素含量:在Sham组,金属元素在心脏含量的排列顺序如下:Mg>Ca>Fe>Zn>Al>Si>Cu>Mn>Se和Cd。各组间比较具有显著差异的是:与Sham组比较,OVX组Zn降低;OVX+Al+E2组Al、Cd、Si、Se均升高;OVX+Al组与OVX组比较,Si升高、Zn降低。OVX+Al+E2组与OVX+Al组比较,Cd、Mn、Se升高。结论雌激素改善卵巢切除及加铝后引起的心脏金属元素含量改变和心肌细胞间隙连接蛋白Cx43重构。Objective To reveal the influence of estrogen replacement threapy on the gap junction connexin 43 （Cx43）expression and content of metal elements in ovariectomy and chronic aluminum toxication rat heart. Methods Forty 6-month-old female SD rats were divided randomly into 4 groups ： group Sham, group OVX, group OVX ＋ A1 and group OVX ＋ A1 ＋ E2 . After 3 months, The content of metalelements in heart was detected by ICP-AES. The Cx43 expressions were observed and determinated by immunohistochemistry and digital image analysis. Results 1. Cx43 positive granules distributed in cell side surface and cytoplasm as well as intercalated disk in atrial myocytes. They typically confined to the site of intercalated disk in ventricular myoeytes in group Sham. In Group OVX, the Cx43 of atrial myocytes were redistributed from cell side surface to the intercalated disk. They were redistributed from the intercalated disk to cell side surface and in cytoplasm in ventricular myocytes. And some Cx43 granules distruibuted irregularly. The Cx43 redistribution of group OVX ＋ A1 was more irregular than that of group OVX. In group OVX ＋ A1 ＋ E2 , the Cx43 distribution of atrial myocytes returned to normal. 2. The amount of Cx43 in all groups was not changed. 3. In sham group, the content of mental elements in heart showed as follows ： Mg 〉 Ca 〉 Fe 〉 Zn 〉 A1 〉 Si 〉 Cu 〉 Mn 〉 Se and Cd;Compared between group OVX and group sham, the content of Zn decreased （ P 〈 0.001 ） ; Compared between group OVX ＋ A1 ＋ E2 and group sham, the content of A1, Cd, Si and Se increased （ P 〈 0.05, P 〈 0.01, P 〈 O.01, P 〈 0.001 ） ;Compared between group OVX ＋ A1 ＋ F2 and group OVX ＋ A1, the content of Cd, Mn and Se increased（ P 〈 0.05, P 〈 0.05, P 〈 0.01）. Conclusion Estrogen can promote the distribution of gap junction connexin43 and change of content of mental elements in ovafiectomy and chronic aluminum toxication rat heart.

关 键 词：CX43 金属元素 雌激素 卵巢切除 心脏 免疫组织化学 大鼠 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]