c-Met在骨骼肌损伤组织肌卫星细胞中的表达  

Expression of c-Met in muscle satellite cells of muscle injury

作　　者：李小雷[1] 黄厚今[1] 杨志明[2] 周光前[2] 解慧琪[2] 邓力[2] 

机构地区：[1]遵义医学院预防医学教研室,遵义563003 [2]四川大学华西医学院生物治疗国家重点实验室干细胞与组织工程研究室,成都610041

出　　处：《解剖学报》2009年第5期780-784,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30670734);四川省科技厅青年基金资助项目(04ZQ026-38)

摘　　要：目的探讨肝细胞生长因子受体（c-Met）在正常和注射心脏毒素后，致肌损伤的肌肉组织中具有再生能力的肌卫星细胞的表达情况；为进一步研究某些肌肉变性疾病，如进行性肌营养不良（DMD）在发病中的分子机制和治疗肌肉退行性变提供参考依据。方法C57雄性小鼠12只，饲养于独立送排风笼具（IVC Cage）实验室。随机分为6组，1-6组左侧股四头肌局部注射心脏毒素（5μg／只），右侧股四头肌为对照，1-6组分别在注射后1d、4d、1周、2周、4周、6周颈椎脱臼处死小鼠，完整分离出股四头肌。浸入4％甲醛溶液中固定，常规包埋切片染色，与对照C57小鼠股四头肌进行组织学和免疫组织化反转录聚合酶链式反应（RT-PCR）比较分析（样本量为10），了解c-Met的原位表达情况。结果免疫组织化学和反转录聚合酶链式反应结果显示，正常小鼠股四头肌中c-Met在肌卫星细胞上仅有少量的表达；与对照比较，注射心脏毒素24h后，表达即明显增加并持续到观察终点的第6周，且其表达量在第1周达到高峰且直到观察期间末（第6周），仍高于正常肌肉组织，其差异有统计学意义（P〈0.05）。结论c-Met可能是肌肉损伤修复过程的关键蛋白。Objective To observe the expression of c-Met in muscle satellite cells in situ of normal and cardiotoxin-intoxicated muscle tissues. Methods Twelve C57 mice were divided into six groups, and raised in IVC laboratory. In the 1st to 6th group, the mice were injectied with cardiotoxin （5/Lg per mouse） in left quadriceps femoris, while their right quadriceps femoris were left for control without any injection. After the mice were sacrificed at different limited time-point. （one day, four days, one week, two weeks, four weeks, six weeks）, the quadriceps femoris were isolated and fixed in 4 percent formaldehyde for 24 hours and then embeded in paraffin for histological, immunohistochemistical and reverse transcription-polymerase chain reaction （RT-PCR） analysis to detect the expression of c-Met in muscle tissues in situ. Results The results of immunohistochemistry and RT-PCR showed that there were few expressions of c-Met in muscle satellite cells of normal muscle tissues. Compared with normal tissues, obvious expression was found in 24 hour-group as well as the sixth week-group. The expression of c-Met peeked in the 1st and expressed continuously untill the 6th week. Conclusion c-Met may be the critical protein in the process of muscle impairment and repaining.

关 键 词：肌卫星细胞 肝细胞生长因子受体 心脏毒素 肌损伤 免疫组织化学 小鼠 

分 类 号：R685.4[医药卫生—骨科学]