BXSB小鼠肝叉状头/翅膀状螺旋转录因子的表达  

Expression of forkhead/winged helix transcription factor in the liver of BXSB mice

作　　者：余立凯[1] 黄安斌[1] 杜戎[1] 沈凌汛[1] 宋优[1] 侯晓华[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院风湿免疫科,武汉430022 [2]华中科技大学同济医学院附属协和医院消化内科,武汉430022

出　　处：《解剖学报》2009年第5期830-833,共4页Acta Anatomica Sinica

摘　　要：目的观察叉状头/翅膀状螺旋转录因子(Foxp3)在BXSB狼疮小鼠肝脏的表达,探索调节性T细胞在狼疮肝损害中的可能作用机制。方法以8周龄正常C57BL/6雄性小鼠作对照,用免疫组织化学及实时PCR方法,检测Foxp3在8周、16周龄雄性BXSB小鼠肝脏的表达。结果正常组C57BL/6小鼠肝组织Foxp3阳性细胞呈深棕黄色,位于肝小叶的肝血窦和(或)窦周隙内,且分布较均匀;8周龄及16周龄BXSB小鼠肝组织内Foxp3染色呈浅棕黄色,明显弱于正常鼠,且分布在界板周边较明显。8周、16周BXSB组小鼠肝组织内Foxp3 mRNA表达水平(0.30±0.04、0.18±0.03)较正常对照组(1.08±0.08)明显降低,16周BXSB组的Foxp3 mRNA表达水平较8周BXSB组明显降低,差异均有统计学意义(P<0.01)。结论BXSB小鼠肝损害的发病机制可能与调节性T细胞的Foxp3分布和表达量下调有关。Objective To present gene and protein expressions of forkhead/winged helix transcription factor （Foxp3） in the liver of BXSB mice and to explore potential roles of regulatory T cell（Treg） in pathogenesis of hepatic lesions in BXSB mice. Methods We examined the liver expression of Foxp3 in 8-and 16-week BXSB male mice using immunohistochemical staining and real-time PCR, and compared with 8-week normal C57BL/6 male mice as a control. Results In immunohistochemistry, Foxp3 staining in normal control mice was pretty strong as dark brown and distributed in hepatic sinusoid and perisinusoidal space, but in 8-and 16-week BXSB mice the staining was weaker as light brown than normal mice and distributed near limiting plate mainly. The mRNA of Foxp3 was significantly down-regulated in 8-and 16-week BXSB mice liver compared to normal controls（0.30±0.04,0.18±0.03 vs 1.08±0.08, P 〈 0.01）, and the Foxp3 expression of 16-week BXSB mice was furthermore downregulated than that of 8-week BXSB mice（P 〈 0.01 ） ; Conclusions Distribution and down-regulation of Foxp3 in Treg may play a role in the pathogenesis of hepatic lesion in BXSB mice.

关 键 词：叉状头/翅膀状螺旋转录因子 红斑狼疮 肝损害 免疫组织化学 BXSB小鼠 

分 类 号：R332[医药卫生—人体生理学] R575.29[医药卫生—基础医学]