Recombinant human PDCD5 protein enhances chemosensitivities of hematologic malignancies  

作　　者：WANG YanFang SHI Lin SONG QuanSheng ZHANG YingMei LOU YaXin ZHENG Yi MA DaLong WANG Ying KE XiaoYan 

机构地区：[1]Department of Hematology, Peking University Third Hospital, Beijing 100191, China [2]Peking University Center for Human Disease Genomics, Beijing 100191, China [3]Department of Medical Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191 China [4]Peking University Medical and Health Analysis Center, Peking University Health Science Center, Beijing 100191, China

出　　处：《Chinese Science Bulletin》2009年第21期3981-3989,共9页

基　　金：Supported by the National High Technology Research and Development Program of China (Grant No. 2006AA02A305);National Natural Science Foundation of China (Grant No. 30671907);Beijing Natural Science Foundation (Grant No. 5072029);Special Fund for Promotion of Ministry of Education (Grant No. 985-2-032-24)

摘　　要：PDCD5 is a novel apoptosis-related gene. Overexpression of PDCD5 facilitates apoptosis in various tumor cells. Here, we investigated the roles of recombinant human PDCD5 (rhPDCD5) protein in chemosensitivities of hematologic malignancies. The rhPDCD5 increased the in vitro cytotoxicity of daunorubicin (DNR), adriamycin (ADM) and As2O3 in three hematologic tumor cell lines. In vivo studies showed that DNR combined with rhPDCD5 significantly suppressed tumor growth of U937 xenograft nude mice compared with DNR alone. In conclusion, rhPDCD5 combined with the chemotherapeutic drug has greater efficacy than chemotherapy alone, and rhPDCD5 is a very promising chemosensitizer.PDCD5 is a novel apoptosis-related gene. Overexpression of PDCD5 facilitates apoptosis in various tumor cells. Here, we investigated the roles of recombinant human PDCD5 （rhPDCD5） protein in chemosensitivities of hematologic malignancies. The rhPDCD5 increased the in vitro cytotoxicity of daunorubicin （DNR）, adriamycin （ADM） and As203 in three hematologic tumor cell lines. In vivo studies showed that DNR combined with rhPDCD5 significantly suppressed tumor growth of U937 xenograft nude mice compared with DNR alone. In conclusion, rhPDCD5 combined with the chemotherapeutic drug has greater efficacy than chemotherapy alone, and rhPDCD5 is a very promising chemosensitizer.

关 键 词：PDCD5 白蛋白 血液病 恶性 重组 肿瘤细胞凋亡 柔红霉素 凋亡相关基因 

分 类 号：Q51[生物学—生物化学] Q512.1