芪术颗粒对肝纤维化模型大鼠Ang-1、Ang-2/Tie-2的影响  

Effects of Qizhu granules on Ang-1,Ang-2/Tie-2 signal transduction pathways in CCl_(4)- induced liver fibrosis rats

作　　者：刘绍能[1] 时磊[1] 李敏[1] 吕鑫霞[1] 阚杰[1] 马继征[1] 陈兰羽[1] 

机构地区：[1]中国中医科学院广安门医院,北京市100053

出　　处：《世界华人消化杂志》2009年第24期2495-2498,共4页World Chinese Journal of Digestology

基　　金：北京市自然科学基金资助项目;No.7072068~~

摘　　要：目的:观察芪术颗粒对肝纤维化模型大鼠肝组织Ang-1、Ang-2/Tie-2的影响.方法:应用CCl4复制大鼠肝纤维化模型,同时给予芪术颗粒灌胃预防,并设立空白对照组、模型对照组、鳖甲软肝片对照组,分别于造模4wk后取大鼠肝组织,实时荧光定量PCR方法检测Ang-1、Ang-2/Tie-2mRNA的表达量.结果:芪术颗粒组Ang-1mRNA表达量明显低于模型组(0.32±0.11vs0.66±0.80,P<0.01),而Ang-2mRNA的表达量明显高于模型组(0.27±0.34vs0.09±0.01,P<0.05).芪术颗粒组和鳖甲软肝片组比较,二者对Ang-1、Ang-2及Tie-2mRNA表达的影响无显著差异.结论:芪术颗粒通过调控Ang-1、Ang-2/Tie-2mRNA的表达,阻断肝窦毛细血管化的形成以减少肝纤维化的产生.AIM： To observe the effects of Qizhu granules on Angiopoietin-1, Angiopoietin-2/Tie-2 signal transduction pathways in CCl4-induced liver fibrosis rats. METHODS： The rats were randomly divided into blank, model, Qizhu granules and tablet of compound Biejia ruangan groups. Liver fibrosis rat models were replicated by CCl4, Qizhu gran- ules and tablets of compound Biejia ruangan were given. And 4 weeks later, liver tissues of each rat were collected and the expression quan- tity of Angiopoietin-1 mRNA, Angiopoietin-2 mRNA and Tie-2 mRNA was detected by real- time fluorescence quantitative PCR. RESULTS： The Ang-1 mRNA expression quan- tity of Qizhu granules was obviously lower than that of the model group （0.32 ± 0.11 vs 0.66 ± 0.80, P 〈 0.01）. The Ang-2 mRNA expression quantity was higher than that of the model group （0.27 ± 0.34 vs 0.09 ± 0.01, P 〈 0.05）. There was no sig- nificant difference in the expression quantity of Ang-1 mRNA, Ang-2 mRNA and Tie-2 mRNA between groups of Qizhu granules and com- pound Biejia ruangan. CONCLUSION： Qizhu granules can alleviate liver fibrosis by intervening liver sinusoidal cap- illarization, and one of its mechanisms is to reg- ulate the expression of Angiopoietin-1 mRNA, Angiopoietin-2 mRNA and Tie-2 mRNA.

关 键 词：芪术颗粒 血管生成素-1 血管生成素-2可溶性酪氨酸激酶受体-2 肝窦毛细血管化 

分 类 号：R285.5[医药卫生—中药学]