人类巨细胞病毒UL147基因在临床低传代分离株中的多态性  

Sequence variability of human cytomegalovirus UL147 genes in clinical isolates

作　　者：何蓉[1] 阮强[1] 齐莹[1] 马艳萍[1] 

机构地区：[1]中国医科大学附属盛京医院病毒室,辽宁省沈阳市110004

出　　处：《世界华人消化杂志》2009年第24期2538-2543,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30700916~~

摘　　要：目的:探讨人类巨细胞病毒(HCMV)UL147序列在临床患儿低传代分离株中的多态性及其与临床疾病的关系.方法:对23株HCMV临床低传代分离株及2例同年龄组健康儿尿液HCMV-DNA采用PCR方法检测,阳性健康儿尿液进行HCMV-UL147基因全序列测定及分析.结果:25株分离株147ORF长度为464-480bp,序列呈现较高的多态性,所有研究的标本中没有序列与Toledo完全一致.序列差异多位于序列的5'端,二级结构预测表现为2种类型,在重要的蛋白质功能区氨基酸序列高度保守.未发现黄疸、小头畸形、先天性巨结肠等不同疾病类型的序列之间的差异.结论:UL147基因可能在HCMV致病中起一定的作用.AIM： To explore the sequence variability of human cytomegalovirus （HCMV） UL147 ORF in clinical isolates and its possible associations with HCMV infection. METHODS： HCMV-UL147 ORF was amplified by PCR assay and sequenced in 23 low-passage isolates and urine from two healthy children. The whole gene sequence of HCMV-UL147 in the urine of healthy children was determined and analyzed. RESULTS： The length of UL147 ORF of 25 isolates was 464-480 bp. Sequencing analysis showed significant strain-specific variability, and none of the sequences was exactly the same with Toledo. The variation was located in the 5＇ end of sequence. The prediction of secondary structure demonstrated two types and the amino acid sequences kept highly conservative in the important functional area of protein. In jaundice, microcephaly and Hirschsprung＇s disease, no sequence differences were found among the dis- ease types. CONCLUSION： HCMV-UL147 might play a role in HCMV infection and subsequent diseases.

关 键 词：人类巨细胞病毒 UL147基因 多态性 

分 类 号：R373[医药卫生—病原生物学] R394[医药卫生—基础医学]