新干特早柚GPAT基因5’端克隆及序列分析  

Cloning and Sequencing Analysis of the 5' Fragment of GPAT Gene from Citrus grandis cv.Xingantezaoyou

作　　者：吴波[1,2] 刘勇[2] 

机构地区：[1]江西中医学院江西省中药种质资源工程技术研究中心,江西南昌330004 [2]江西农业大学农学院,江西南昌330045

出　　处：《江西农业大学学报》2009年第5期915-918,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金资助项目(30070528);江西农业大学博士启动基金项目(1791)

摘　　要：采用5’-RACE技术从新干特早柚(Citrus grandiscv.Xingantezaoyou)叶片中克隆出一条长832 bp的GPAT基因5’端cDNA片段(GenBank登录号:DQ193970)。序列分析结果表明:该cDNA片段序列与其它科属植物的GPAT基因序列的同源性差异较大,除与同类植物——温州蜜柑(Citrus unshiu)的同源性高达98.9%,与红花(Carthamus tinctorius)、拟南芥(Arabidopsis thaliana)、非洲油棕榈(Elaeis guineensis)、豌豆(Pisum sati-vum)、蚕豆(Vicia faba)的同源性分别为62.5%、54.6%、48.2%、47.4%、46.9%;其所编码的氨基酸序列与红花、拟南芥、非洲油棕榈、豌豆、辣椒的同源性分别为51.6%、60%、45.1%、48%、52.3%,与细菌(Bacillusamyloliquefaciens)的同源性仅为18.9%,说明GPAT基因的氨基酸序列具有种属特异性。Citrus maxima cv. Xingantezaoyou＇ s GPAT gene of 5＇ end fragment was isolated from the leaf by means of 5 ＇ - RACE technology. The cDNA fragment is 832 bp long （ GenBank Accession No： DQ193970）. The nucleotide sequence analysis showed that these sequences of GPAT 5＇ end fragment are greatly different from those of other plants. The best nucleotide similarity to Citrus unshiu was 98.9% but those Carthamus tinctorius , Arabidopsis thaliana ,Elaeis guineensis , Pisum sativum and Vicia faba were 62.5% ,54.6%, 48.2% ,47.4% and 46.9% respectively. The deduce amino acids sequence analysis showed the similarity to Carthamus tinctorius ,Arabidopsis thaliana, Elaeis guineensis ,Pisurn sativum and Capsicum annuum were 51.6%, 60% ,45.1% ,48% and 52.3%. The amino acid sequences of GPAT gene has genera specificity because the similarity of amino acid sequences to Bacillus amyloliquefaciens is only 18.9%.

关 键 词：新干特早柚 GPAT RACE 基因克隆 序列分析 

分 类 号：Q785[生物学—分子生物学] S666.3[农业科学—果树学]