文蛤多肽体外对人肺癌A549细胞的抑制作用  

Effect of peptide from Meretrix meretrix on the human lung adenocarcinoma A549 cells in vitro

作　　者：康劲翮[1] 任跃明 李华亮[1] 范成成[1] 张剑[1] 邱乒乒[1] 陈清西[1] 

机构地区：[1]厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室厦门大学生命科学学院,福建厦门361005 [2]中国药典委员会,北京100061

出　　处：《台湾海峡》2009年第4期477-481,共5页Journal of Oceanography In Taiwan Strait

基　　金：厦门市科技计划资助项目(3502Z20063021);厦门大学科技创新工程基金资助项目(XDKJCX20043001)

摘　　要：我们已从文蛤肉中提取得到1种具有抗癌活性的多肽,命名为Mer2.本文报道Mer2对体外培养的肺癌A549细胞的生物学效应.采用溴化二苯偶氮盐(MTT)法检测Mer2对肺癌A549细胞的抑制效果,并应用细胞形态观察法、Hoechst荧光染色和流式细胞术等方法,探讨了Mer2对细胞形态、细胞周期的影响.结果表明,Mer2对体外培养的肺癌A549细胞的生长有较强的抑制作用,对作用含量和时间均具有一定的依赖性.Mer2使细胞形态发生明显变化,明显地出现凋亡峰,但并没有出现明显细胞周期阻滞现象.这表明文蛤多肽可能通过诱导细胞凋亡从而抑制癌细胞的生长.An anticancer peptide named as mer2 was purified from Meretrix meretrix. In this paper, we investigated its biological effect on the lung adenocarcinoma A549 ceils. The effect of Mer2 on the growth of A549 cells was detected by MTT assay, and the morphology and the cell-cycle of cells were detected by optical microscope, Hoechst staining and flow cytometry. The results showed that Mer2 inhibited the growth of A549 ceils in a dose-and time- dependent manner and cell morphology changed distinctly, showing an obvious apoptotsis properties. It indicated that the inhibitory effect of the peptide on cells had a relationship with the cell apoptotsis.

关 键 词：海洋生物学 文蛤多肽 抗癌 肺癌细胞 凋亡 

分 类 号：Q247[生物学—细胞生物学]