纳米银体外抗HIV活性的研究  

STUDY ON ANTI-HIV ACTIVITY OF SILVER NANOPARTICLES IN VITRO

作　　者：王云华[1,2] 黄宁[2] 于增国[1] 周广运[3] 郑永唐[2] 郑丛龙[1] 

机构地区：[1]大连大学医学院,大连116622 [2]中国科学院昆明动物研究所动物模型与人类疾病机理重点实验室,分子免疫药理学实验室 [3]大连大学环化学院

出　　处：《现代预防医学》2009年第21期4123-4126,共4页Modern Preventive Medicine

基　　金：辽宁省自然科学研究项目(20062133);国家自然科学基金面上项目(30870681);辽宁省教育厅资助项目;863计划(2006AA020602);973计划(2009CB522306);中国科学院重要方向项目(KSCX1-YW-R-24);云南省科技计划项目(2007BC006)

摘　　要：[目的]制备纳米银颗粒并检测其抗人类免疫缺陷病毒(HIV)活性及其体内外毒性,探讨纳米银体外抗HIV的作用机制。[方法]在体外培养C8166细胞中加入不同浓度的纳米银,检测其体外细胞毒性CC50;不同病毒株感染细胞,同时加入不同浓度的纳米银,孵育后检测细胞病变及病毒抗原,检测纳米银对HIV的抑制作用。纳米银滴鼻处理小鼠,检测小鼠体重变化,并进行病理切片,观察纳米银滴鼻处理小鼠的肝、肾及肺组织的病理变化。[结果]纳米银颗粒对C8166细胞毒性的CC50为105.73μg/ml,抑制HIV实验株HIV-1IIIB,HIV-2CBL-20诱导细胞病变的EC50分别为26.56μg/ml和35.47μg/ml;抑制HIV-1IIIBp24抗原产生的EC50为9.80μg/ml;直接杀病毒作用的EC50为12.08μg/ml。纳米银经滴鼻处理的小鼠,其体重及肝、肾及肺组织切片与对照组比较,均无明显改变。[结论]纳米银有较好的抗HIV活性,作用机制可能主要通过直接杀病毒来抑制HIV的复制;纳米银在呼吸道黏膜局部应用无明显的毒副作用,作为黏膜表面抗病毒药物,值得进一步深入研究。[Objective] To analyze the anti-HIV activities and cytotoxicity of silver nanoparticles. [Methods] C8166 cells were infected with HIV-1 or HIV-2 strains. The cytopathic effect （CPE） was measured by counting the number of syncytium. The p24 in the supernatant was examined by ELISA. To examine the virucide activity. HIV-1ⅢB was incubated with silver nanoparticles for 1 h and virus was added. Syncytia were scored after 72h. Cytotoxicity to C8166cells was assessed by MTr method. To test toxicology, in vivo, the mice were given nasal dropping of silver nanoparticles. Mice weight and histological section of liver, lung and renal ussues were examined. [Results] Silver nanoparticles suppressed syncytium formation in HIV- 1 IIIB or HIV-2CBL-20 infected C8166 cell hne with EC50 value of 26.56 μg/ml and 35.47 μg/ml, respectively. It also reduced p24 production in HIV-1ⅢB acute infected C8166 with EC50 value of 9.80 μg/ml. Further, silver nanopartieles showed virueide aetivity with EC50 value of 12.08 μ g/ml. Nasal dropping of silver nanoparticles showed effect neither on mice＇ s weight nor on liver, lung and renal tissues. [Conclusion] Silver nanoparticles can inhibit HIV-1 and HIV-2 replication in vitro by its virueide activity. No acute toxicity is found on the mice treated by nasal dropping of silver nanoparticles.

关 键 词：人类免疫缺陷病毒 纳米银 抗病毒 毒副作用 

分 类 号：R512.91[医药卫生—内科学]