小麦叶绿体DNA的提取与酶切  

Purification and Enzymolysis of Chlorplast DNA of Wheat

作　　者：周晋[1] 刘云英[1] 李维琪[1] 

机构地区：[1]中科院新疆化学所

出　　处：《新疆大学学报（自然科学版）》1998年第3期58-60,共3页Journal of Xinjiang University(Natural Science Edition)

摘　　要：研究小麦叶绿体ＤＮＡ，需要快速，简便地获得质纯、量大的ｃｔＤＮＡ．将高离子强度和抗坏血酸同时引入缓冲系统用于提取小麦ｃｔＤＮＡ，利用高离子强度对ＤＮＡ与蛋白质之间的静电吸引造成裹胁损失的阻碍作用，及抗坏血酸提供低ｐＨ环境抑制酶活性等作用，得到较好的产率和纯度，并可得到清晰的限制性内切酶图谱．The more effectively and more rapidly method was used to purify ctDNA of wheat in this paper.The high ionic strength and ascorbic acid were used to get higher yield and better purity.A clearly restriction enzyme mapping would be obtained.High ionic strenth would decrease the electrostatic interaction,and decrease sticking loss that was led by electrostatic interaction between the DNA and protein.Ascorbic acid would provide a low pH condition to restrain the activity of enzyme.

关 键 词：叶绿体DNA 提取 小麦 酶切图谱 

分 类 号：S512.1[农业科学—作物学]