蒺藜皂苷对动脉粥样硬化大鼠动脉壁ICAM-1、VCAM-1、PPARα、PPARγ基因表达的影响  

Effects of Tribu Saponin from Tribulus terrestris on Gene Expression of ICAM-1,VCAM-1,PPARα and PPARγ in Artery Vessels of Atherosclerotic Rats

作　　者：石昌杰[1] 瞿伟菁[1] 高娟[2] 

机构地区：[1]华东师范大学生命科学学院,上海200062 [2]复旦大学生命科学学院,上海200433

出　　处：《天然产物研究与开发》2009年第5期761-765,共5页Natural Product Research and Development

基　　金：国家自然科学基金(30370158)

摘　　要：观察全草蒺藜皂苷(tribu saponin fromTribulus terrestris,STT)对实验性动脉粥样硬化(atherosclerosis,AS)大鼠动脉壁中ICAM-1、VCAM-1、PPARα和PPARγ基因表达的影响,以探讨STT抗AS的机制。应用高脂饲料饮食配合注射维生素D3建立SD大鼠AS模型,并设立正常组、模型组、辛伐他汀组和蒺藜皂苷低、中、高剂量组。采用半定量RT-PCR的方法检测各组动物动脉壁中ICAM-1、VCAM-1、PPARα和PPARγ基因的表达,分析造模及各给药大鼠ICAM-1、VCAM-1、PPARα和PPARγ基因表达的变化。与正常组相比,模型组ICAM-1和VCAM-1基因的表达量明显增加(P<0.01),而PPARα和PPARγ基因的表达量明显降低(P<0.01);与模型组相比,辛伐他汀及各STT药均能降低ICAM-1和VCAM-1基因的表达量(P<0.01～P<0.05),并能增加PPARα和PPARγ基因的表达量(P<0.01)。提示STT能下调实验性AS大鼠动脉壁ICAM-1和VCAM-1基因的表达及上调PPARα和PPARγ基因表达,这可能是STT抗AS的作用机制之一。To investigate the effects of tribu saponin from Tribulus terrestris（STr） on the gene expression of ICAM-1, VCAM-1 ,PPARα and PPARγ in artery vessels of atherosclerotic rats,the model rats of atheresclerosis were established by feeding with high cholesterol diet and injecting with vitamin D3. The rats were divided into normal group, model group,simvastatin group and 3 groups with STT. Semi-quantitative RT-PCR was used to detect ICAM-1, VCAM-1, PPARα and PPARγ gene expression in artery vessels. The changes of gene expression of ICAM-1, VCAM-1, PPARα and PPARγ in artery vessels in each group were analyzed. The results showed that ICAM-1 and VCAM-1 gene expression levels in the model group were significantly higher than those of normal group （P 〈0.01 ） ,while the PPARα, PPARγ gene expression in the model group were significantly lower than those of normal group （ P 〈 0.01 ） ; Compared with model group,the ICAM-1 and VCAM-1 gene expression levels in each medicated group were significantly lower （P 〈0.01- P 〈0.05） and the PPARα and PPARγ gene expression levels in each medicated group were significantly higher（ P 〈 0. 01 ）. SIT can down regulate the gene expression of ICAM-1 ,VCAM-1and up regulate the gene expression of PPARα, PPAR3, in artery vessels of arteresclerotic rats, which may account for the anti-arteriosclerosis effects of STT.

关 键 词：蒺藜皂苷 动脉粥样硬化 细胞间黏附分子-1 血管细胞黏附分子-1 过氧化物酶增殖体激活受体 

分 类 号：R285[医药卫生—中药学] Q946.91[医药卫生—中医学]