桦木酮酸对肿瘤细胞SGC-7901、HepG-2及S_(180)荷瘤小鼠肿瘤细胞的影响  

Effects of Betulonic Acid on SGC-7901,HepG-2 and Mice of Bearing S180 Tumor Cells

作　　者：张秀娟[1,2] 李宏伟[1,2] 季宇彬[1,2] 方桂珍[3] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076 [2]哈尔滨商业大学药物研究所抗肿瘤天然药物教育部工程研究中心,哈尔滨150076 [3]东北林业大学生物质材料科学与技术教育部重点实验室,哈尔滨150040

出　　处：《天然产物研究与开发》2009年第5期766-770,共5页Natural Product Research and Development

基　　金：黑龙江省自然科学基金项目(C200604);黑龙江省教育厅骨干教师项目

摘　　要：为研究桦木酮酸体外对SGC-7901、HepG-2及体内对S180荷瘤小鼠的影响,采用MTT与肿瘤细胞集落形成能力实验观察桦木酮酸对HepG-2和SGC-7901作用。通过抑瘤率观察其对S180荷瘤小鼠的影响;HE染色观察其对S180肿瘤细胞形态学的影响。结果显示:桦木酮酸可抑HepG-2和SGC-7901细胞的生长,MTT与肿瘤集落形成能力实验测得其IC50分别为68.14和110.77μmol/L。连续给药8 d后,150和75 mg/kg/d剂量的桦木酮酸对S180的抑制率分别为68.3%和41.5%(P<0.05);HE染色发现肿瘤细胞表现较为明显的胀亡现象:细胞淡染、细胞体积增大,细胞核结构相对完整。提示桦木酮酸体内、外对肿瘤细胞均有一定的抑制作用,其作用可能与其引起肿瘤细胞胀亡有关。To study the effects of betulonic acid on SGC-7901, HepG-2 in vitro and mice of bearing S180 tumor cells in vivo ,the effects of betulonic acid on tumor cell of SGC-7901 and HepG-2 were observed by MTTand colony formation. The morphocytology of S180 tumor cells was observed by HE stain. It has been found that betulonic acid can inhibit the growth of tumor cell SGC-7901 and HepG-2 in vitro studies. Their IC50 were 68.14 and 110.77 μmol/L respectively. The inhibition rate on S180 tumor cells were 68.3% and 41.5%, respectively after being administered orally for 8 consecutive days at 150 and 75 mg/kg/d （P 〈0.05）. The morphocytology of Sts0 tumor cells had exhibited an obvious oncosis,such as cell light staining,increased cell volume and the intact nucleus structure. Betalonic acid has definite action on tumor ceils and the mechanism may be induced cell oncosis.

关 键 词：桦木酮酸 肿瘤细胞 MTT 集落形成 胀亡 

分 类 号：R285[医药卫生—中药学] Q946.91[医药卫生—中医学]