高速逆流色谱法分离凤尾草中的芹菜素和木犀草素(英文)  

Isolation and Purification of Apigenin and Luteolin from Pteris multifida Poir.by High-speed Counter-current Chromatography

作　　者：欧阳丹薇[1] 孔德云[1] 许海燕[1] 倪翔[1] 陈佳[1] 杨培明[1] 

机构地区：[1]上海医药工业研究院中药室,上海200040

出　　处：《天然产物研究与开发》2009年第5期822-825,839,共5页Natural Product Research and Development

摘　　要：建立了高速逆流色谱分离纯化芹菜素和木犀草素的方法。两相溶剂系统为氯仿-甲醇-水(4∶3∶2),上相为固定相,下相为流动相进行洗脱。从100 mg粗提物中一步分离得到17.0 mg芹菜素和22.5 mg木犀草素,经高效液相色谱分析,纯度分别为92%和96%。其化学结构由1H和13C NMR鉴定。High-speed counter-current chromatography （HSCCC）was applied to isolate and purify bioactive compounds from Pteris multifida Poir. Two flavonoids apigenin and luteolin were successfully isolated and purified by using twophase solvent system, which was composed of chloroform-methanol-water （4： 3： 2, v/v/v） in head to tall elution mode. Consequently, 17.0 mg of apigenin and 22.5 mg of luteolin were obtained from 100 mg crude sample with purities of 92% and 96% ,respectively,as determined by HPLC. The chemical structures were identified by 1H and 13C NMR.

关 键 词：高速逆流色谱 凤尾草 黄酮 芹菜素 木犀草素 

分 类 号：R284.2[医药卫生—中药学] Q946.91[医药卫生—中医学]