反相高效液相色谱法同时测定麻黄中麻黄碱和伪麻黄碱  

SIMULTANEOUS DETERMINATION OF EPHEDRINE AND PSEUDOEPHEDRINE IN EPHEDRAE HERBA BY HPLC

作　　者：杨文远[1,2] 杨美君[1,2] 王天勇[1,2] 

机构地区：[1]宁夏大学化学与化工系 [2]宁夏自治区人民医院

出　　处：《宁夏大学学报（自然科学版）》1998年第3期253-254,共2页Journal of Ningxia University(Natural Science Edition)

基　　金：宁夏区科委基金

摘　　要：用反相高效液相色谱法分离并测定了麻黄中麻黄碱和伪麻黄碱，建立了该中药中麻黄碱、伪麻黄碱分离、测定的色谱方法．色谱条件：ＯＤＳ柱，甲醇－２０ｍｍｏｌ／Ｌ磷酸二氢钾－三乙胺（９９１０．２Ｖ／Ｖ）为流动相，紫外检测波长为２１０ｎｍ．本研究为麻黄的质量评价提供了科学依据．Ephedrine and pseudoephedrine in ephedrae herba are separated and determined by RP HPLC. A chromatographic method for the separation and determination of ephedrine and pseudoephedrine in this Chinese medicinal herb was established.Chromatographic conditions:ODS colum with methanol 20 mmol/L potassium dihydrogen phosphate triethylamine(9∶91∶0.2 V/V ) as mobile phase,and UV detection at 210nm.A scientific basis for quality evaluation of ephedrae herba is provided.

关 键 词：麻黄 麻黄碱 伪麻黄碱 高效液相色谱 中草药 

分 类 号：R284.1[医药卫生—中药学]